STUB1 Recombinant Rabbit Monoclonal Antibody [JG38-22] (ET7108-65)
Catalog# ET7108-65
Rabbit Monoclonal to STUB1
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WB
-
IF-Cell
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IHC-P
-
IP
-
FC
-
Human
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Mouse
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Rat
Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: HEK-293 cell lysate (15 µg/Lane)
Lane 3: Human brain tissue lysate (30 µg/Lane)
Predicted band size: 35 kDa
Observed band size: 34 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-65) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
STUB1 Recombinant Rabbit Monoclonal Antibody [JG38-22] (ET7108-65)
Immunogen
Synthetic peptide within human stub1 aa 10-59 / 303.
Host
Rabbit
Positive Control
HeLa cell lysate, HEK-293 cell lysate, human brain tissue lysate, human heart tissue, mouse heart tissue, rat heart tissue, 293T, LOVO, MCF-7, SH-SY5Y.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JG38-22
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
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IP
-
Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 35 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
STUB1
SYNONYMS
Antigen NY CO 7 antibody;Antigen NY-CO-7 antibody;C terminus of Hsp70-interacting protein antibody;Carboxy terminus of Hsp70 interacting protein antibody;Carboxy terminus of Hsp70-interacting protein antibody;Carboxy terminus of Hsp70p interacting protein antibody;CHIP antibody;CHIP_HUMAN antibody;CLL associated antigen KW 8 antibody;CLL-associated antigen KW-8 antibody;E3 ubiquitin protein ligase CHIP antibody;E3 ubiquitin-protein ligase CHIP antibody;Heat shock protein A binding protein 2 (c terminal) antibody;HSPABP2 antibody;NY CO 7 antibody;PP1131 antibody;SDCCAG7 antibody;Serologically defined colon cancer antigen 7 antibody;STIP1 homology and U Box containing protein 1 antibody;STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase antibody;STIP1 homology and U box-containing protein 1 antibody;STUB 1 antibody;STUB1 antibody;UBOX 1 antibody;UBOX1 antibody
TISSUE SPECIFICITY
Expressed in differentiated myotubes (at protein level). Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.
POST-TRANSLATIONAL MODIFICATION
Monoubiquitinated at Lys-2 following cell stress by UBE2W, promoting the interaction with ATXN3 (By similarity). Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2.
SUBCELLULAR LOCATION
Nucleus. Cytoplasm.
FUNCTION
CHIP (carboxy terminus of HSP 70-interacting protein), also designated STIP1 homology and U-box containing protein 1, HSPABP2, NY-CO-7, SDCCAG7 and STUB1, is a cytoplasmic E3 ubiquitin ligase that influences protein ubiquitylation. CHIP interacts with Smad1/Smad4 and blocks BMP signaling through the ubiquitin-mediated degradation of Smad proteins. CHIP controls both association of Hsp70/Hsp90 chaperones with ErbB2 and down-regulation of ErbB2 induced by inhibitors of Hsp90. A 1.3-kb transcript is most abundant in striated muscle (heart and skeletal muscle), with lower expression in pancreas and brain.
CITATIONS
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STUB1-mediated ubiquitination regulates the stability of GLUD1 in lung adenocarcinoma
