Lane 1: 293 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: SK-Br-3 cell lysate
Recombinant Rabbit monoclonal primary
STUB1 Recombinant Rabbit Monoclonal Antibody [JG38-22] (ET7108-65)
Recombinant protein within human stub1 aa 1-200.
293 cell lysate, MCF-7 cell lysate, SK-Br-3 cell lysate, 293T, LOVO, MCF-7, rat epididymis tissue, human colon carcinoma tissue, human placenta tissue, mouse kidney tissue, SH-SY5Y.
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
Antigen NY CO 7 antibody; Antigen NY-CO-7 antibody; C terminus of Hsp70-interacting protein antibody; Carboxy terminus of Hsp70 interacting protein antibody; Carboxy terminus of Hsp70-interacting protein antibody; Carboxy terminus of Hsp70p interacting protein antibody; CHIP antibody; CHIP_HUMAN antibody; CLL associated antigen KW 8 antibody; CLL-associated antigen KW-8 antibody; E3 ubiquitin protein ligase CHIP antibody; E3 ubiquitin-protein ligase CHIP antibody; Heat shock protein A binding protein 2 (c terminal) antibody; HSPABP2 antibody; NY CO 7 antibody; PP1131 antibody; SDCCAG7 antibody; Serologically defined colon cancer antigen 7 antibody; STIP1 homology and U Box containing protein 1 antibody; STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase antibody; STIP1 homology and U box-containing protein 1 antibody; STUB 1 antibody; STUB1 antibody; UBOX 1 antibody; UBOX1 antibody
Expressed in differentiated myotubes (at protein level). Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.
Monoubiquitinated at Lys-2 following cell stress by UBE2W, promoting the interaction with ATXN3 (By similarity). Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2.
CHIP (carboxy terminus of HSP 70-interacting protein), also designated STIP1 homology and U-box containing protein 1, HSPABP2, NY-CO-7, SDCCAG7 and STUB1, is a cytoplasmic E3 ubiquitin ligase that influences protein ubiquitylation. CHIP interacts with Smad1/Smad4 and blocks BMP signaling through the ubiquitin-mediated degradation of Smad proteins. CHIP controls both association of Hsp70/Hsp90 chaperones with ErbB2 and down-regulation of ErbB2 induced by inhibitors of Hsp90. A 1.3-kb transcript is most abundant in striated muscle (heart and skeletal muscle), with lower expression in pancreas and brain.