PRODUCT CODE: ET1612-53

Stromal interaction molecule 1 Recombinant Rabbit Monoclonal Antibody [SD0814] (ET1612-53)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

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+
Western blot analysis of STIM1 on different lysates using anti-STIM1 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: K562                <br />
Lane 2: HepG2
  • Western blot analysis of STIM1 on different lysates using anti-STIM1 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: K562                <br />
Lane 2: HepG2
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-STIM1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-STIM1 antibody. Counter stained with hematoxylin.
Western blot analysis of STIM1 on different lysates using anti-STIM1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562
Lane 2: HepG2

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Stromal interaction molecule 1 Recombinant Rabbit Monoclonal Antibody [SD0814] (ET1612-53)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, HepG2, human spleen tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD0814

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

100 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Stromal interaction molecule 1

SYNONYMS

D11S4896E antibody; GOK antibody; OTTHUMP00000164512 antibody; OTTHUMP00000229140 antibody; OTTHUMP00000230742 antibody; SIM antibody; STIM 1 antibody; STIM1 antibody; Stim1 stromal interaction molecule 1 antibody; STIM1_HUMAN antibody; STIM1L antibody; Stromal interaction molecule 1 antibody

TISSUE SPECIFICITY

Ubiquitously expressed in various human primary cells and tumor cell lines.

POST-TRANSLATIONAL MODIFICATION

Glycosylation is required for cell surface expression.; Phosphorylated predominantly on Ser residues.

SUBCELLULAR LOCATION

Cell membrane, Endoplasmic reticulum membrane, Cytoplasm, Sarcoplasmic reticulum.

FUNCTION

Ca2+ influx is essential for a variety of cellular functions including, secretion and transcription. Stromal interaction molecule 1 (Stim1) is a ubiquitously expressed cell surface transmembrane glycoprotein that plays a role in medi-ating Ca2+ influx following the depletion of intracellular Ca2+ stores. Stim1 functions in the endoplasmic reticulum (ER) where it acts as a Ca2+ sensor via its EF-hand domain which causes large conformational changes. When Ca2+ levels drop, Stim1 translocates from the ER to the plasma membrane, where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit, TMEM142A/Orai1. Stim2 is a potent inhibitor of Stim1-mediated store-operated calcium (SOC) entry. Stim1 is implicated in tumor growth suppression and stromal-hematopoietic cell interactions.