PRODUCT CODE: R1306-1

Stella Rabbit Polyclonal Antibody (R1306-1)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

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+
Western blot analysis on cell lysates using anti-Stella rabbit polyclonal antibodies.
  • Western blot analysis on cell lysates using anti-Stella rabbit polyclonal antibodies.
  • Immunohistochemical analysis of paraffin-embedded mouse teratoma tissue using anti-Stella rabbit polyclonal antibody.
Western blot analysis on cell lysates using anti-Stella rabbit polyclonal antibodies.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Stella Rabbit Polyclonal Antibody (R1306-1)

Immunogen

Peptide

Host

Rabbit

Positive Control

F9, NCCIT, mouse teratoma, MEF-1

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

17 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

  • IHC-P

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Stella

SYNONYMS

Compaction associated protein 1 antibody; Developmental pluripotency associated 3 antibody; Developmental pluripotency associated protein 3 antibody; Developmental pluripotency-associated protein 3 antibody; Dppa3 antibody; DPPA3_HUMAN antibody; PGC7 antibody; Stella related protein antibody; Stella-related protein antibody

TISSUE SPECIFICITY

Low expression in testis, ovary and thymus. Expressed in embryonic stem and carcinoma cells. Highly expressed in testicular germ cell tumors.

DEVELOPMENTAL STAGE

Expressed in fetal ovary.

SUBCELLULAR LOCATION

Cell nucleus, cytoplasm

FUNCTION

Stella is a primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. It participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. It is important for the totipotent/pluripotent states continuing through preimplantation development.