PRODUCT CODE: ET1701-42

STAT4 Recombinant Rabbit Monoclonal Antibody [JJ08-85] (ET1701-42)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

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Western blot analysis of STAT4 on Daudi cells lysates using anti-STAT4 antibody at 1/1,000 dilution.
  • Western blot analysis of STAT4 on Daudi cells lysates using anti-STAT4 antibody at 1/1,000 dilution.
  • ICC staining STAT4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining STAT4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining STAT4 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-STAT4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-STAT4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-STAT4 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with STAT4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of STAT4 on Daudi cells lysates using anti-STAT4 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

STAT4 Recombinant Rabbit Monoclonal Antibody [JJ08-85] (ET1701-42)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Daudi, MCF-7, Hela, SW480, human colon cancer tissue, human breast carcinoma tissue, human pancreas tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ08-85

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

86 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

STAT4

SYNONYMS

Signal transducer and activator of transcription 4 antibody; SLEB11 antibody; STAT4 antibody; STAT4_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the transcription factor STAT family.

POST-TRANSLATIONAL MODIFICATION

Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity (By similarity).

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.