PRODUCT CODE: ET1606-39

STAT1 Recombinant Rabbit Monoclonal Antibody [SJ01-89] (ET1606-39)

  • Recombinant

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

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+
Western blot analysis of STAT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-39, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: 293 cell lysate<br />
Lane 2: NIH/3T3 cell lysate<br />
Lane 3: A431 cell lysate<br />
Lane 4: MCF-7 cell lysate
  • Western blot analysis of STAT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-39, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: 293 cell lysate<br />
Lane 2: NIH/3T3 cell lysate<br />
Lane 3: A431 cell lysate<br />
Lane 4: MCF-7 cell lysate
  • Flow cytometric analysis of STAT1 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1606-39, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of STAT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-39, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293 cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: A431 cell lysate
Lane 4: MCF-7 cell lysate

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

STAT1 Recombinant Rabbit Monoclonal Antibody [SJ01-89] (ET1606-39)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293 cell lysate, NIH/3T3 cell lysate, A431 cell lysate, MCF-7 cell lysate, MCF-7.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SJ01-89

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

83/87 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

STAT1

SYNONYMS

Signal transducer and activator of transcription 1 91kD antibody; CANDF7 antibody; DKFZp686B04100 antibody; IMD31A antibody; IMD31B antibody; IMD31C antibody; ISGF 3 antibody; ISGF-3 antibody; OTTHUMP00000163552 antibody; OTTHUMP00000165046 antibody; OTTHUMP00000165047 antibody; OTTHUMP00000205845 antibody; Signal transducer and activator of transcription 1 91kDa antibody; Signal transducer and activator of transcription 1 antibody; Signal transducer and activator of transcription 1, 91kD antibody; Signal transducer and activator of transcription 1-alpha/beta antibody; STAT 1 antibody; Stat1 antibody; STAT1_HUMAN antibody; STAT91 antibody; Transcription factor ISGF 3 components p91 p84 antibody; Transcription factor ISGF-3 components p91/p84 antibody

SEQUENCE SIMILARITIES

Belongs to the transcription factor STAT family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on tyrosine and serine residues in response to a variety of cytokines/growth hormones including IFN-alpha, IFN-gamma, PDGF and EGF. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Upon EGF stimulation, phosphorylation on Tyr-701 (lacking in beta form) by JAK1, JAK2 or TYK2 promotes dimerization and subsequent translocation to the nucleus. Growth hormone (GH) activates STAT1 signaling only via JAK2. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylation on Ser-727 by several kinases including MAPK14, ERK1/2 and CAMKII on IFN-gamma stimulation, regulates STAT1 transcriptional activity. Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS. Phosphorylation on Ser-727 by PRKCD induces apoptosis in response to DNA-damaging agents. Phosphorylated on tyrosine residues when PTK2/FAK1 is activated; most likely this is catalyzed by a SRC family kinase. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates interferon-mediated signaling. Upon viral infection or IFN induction, phosphorylation on Ser-708 occurs much later than phosphorylation on Tyr-701 and is required for the binding of ISGF3 on the ISREs of a subset of IFN-stimulated genes IKBKE-dependent. Phosphorylation at Tyr-701 and Ser-708 are mutually exclusive, phosphorylation at Ser-708 requires previous dephosphorylation of Tyr-701.; Sumoylated with SUMO1, SUMO2 and SUMO3. Sumoylation is enhanced by IFN-gamma-induced phosphorylation on Ser-727, and by interaction with PIAS proteins. Enhances the transactivation activity.; ISGylated.; Mono-ADP-ribosylated at Glu-657 and Glu-705 by PARP14; ADP-ribosylation prevents phosphorylation at Tyr-701. However, the role of ADP-ribosylation in the prevention of phosphorylation has been called into question and the lack of phosphorylation may be due to sumoylation of Lys-703.; Monomethylated at Lys-525 by SETD2; monomethylation is necessary for phosphorylation at Tyr-701, translocation into the nucleus and activation of the antiviral defense.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.