STAM1 Rabbit Polyclonal Antibody (HA500430)
Catalog# HA500430
Rabbit Polyclonal to STAM1
Tested for
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IF-Cell
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IHC-P
Reacts with
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Human
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Rat
Immunocytochemistry analysis of Siha cells labeling STAM1 with Rabbit anti-STAM1 antibody (HA500430) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 30 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at 37℃. Cells were then incubated with Rabbit anti-STAM1 antibody (HA500430) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, Red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution.
Cells were fixed in 4% paraformaldehyde for 30 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at 37℃. Cells were then incubated with Rabbit anti-STAM1 antibody (HA500430) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, Red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution.