Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of SSX2IP on different lysates with Rabbit anti-SSX2IP antibody (HA721065) at 1/500 dilution.
Lane 1: Daudi cell lysate
Lane 2: K562 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 80/90 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721065) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ADIP antibody;ADIP_HUMAN antibody;Afadin and alpha actinin binding protein antibody;Afadin DIL domain interacting protein antibody;Afadin DIL domain-interacting protein antibody;Afadin- and alpha-actinin-binding protein antibody;FLJ10848 antibody;KIAA0923 antibody;MGC75026 antibody;OTTHUMP00000011574 antibody;SSX 2 interacting protein antibody;SSX2 interacting protein antibody;SSX2-interacting protein antibody;SSX2IP antibody;Synovial sarcoma X breakpoint 2 interacting protein antibody"
Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin. Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling. Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome. Involved in ciliogenesis. It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia.
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.