SRPK1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH08-26] (HA751217)
Catalog# HA751217
Rabbit Monoclonal to SRPK1
Tested for
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WB
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IF-Cell
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IHC-P
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IF-Tissue
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FC
-
IP
Reacts with
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Human
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Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of SRPK1 on different lysates with Rabbit anti-SRPK1 antibody (HA751217) at 1/5,000 dilution.
Lane 1: NCI-H226 cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: Caco-2 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Lane 5: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 6: BxPC-3 cell lysate (20 µg/Lane)
Lane 7: Mouse testis tissue lysate (40 µg/Lane)
Predicted band size: 74 kDa
Observed band size: 100 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751217) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: NCI-H226 cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: Caco-2 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Lane 5: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 6: BxPC-3 cell lysate (20 µg/Lane)
Lane 7: Mouse testis tissue lysate (40 µg/Lane)
Predicted band size: 74 kDa
Observed band size: 100 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751217) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.