Src Family Antibody Sampler Kit (HAK21092)
Catalog# HAK21092
Reacts with
-
Human
Western blot analysis of Src on different lysates with Rabbit anti-Src antibody (ET1702-03) at 1/1,000 dilution.
Lane 1: SK-OV-3 cell lysate, 20 µg/Lane
Lane 2: A549 cell lysate, 20 µg/Lane
Lane 3: NIH/3T3 cell lysate, 20 µg/Lane
Lane 4: 4T1 cell lysate, 20 µg/Lane
Lane 5: PC-12 cell lysate, 20 µg/Lane
Lane 6: C6 cell lysate, 20 µg/Lane
Lane 7: COS-1 cell lysate, 20 µg/Lane
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 16 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-03) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: SK-OV-3 cell lysate, 20 µg/Lane
Lane 2: A549 cell lysate, 20 µg/Lane
Lane 3: NIH/3T3 cell lysate, 20 µg/Lane
Lane 4: 4T1 cell lysate, 20 µg/Lane
Lane 5: PC-12 cell lysate, 20 µg/Lane
Lane 6: C6 cell lysate, 20 µg/Lane
Lane 7: COS-1 cell lysate, 20 µg/Lane
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 16 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-03) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.