Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
SPATA13 Rabbit Polyclonal Antibody (ER1902-32)
Immunogen
Recombinant protein within human spata13 aa 423-572 / 652.
Host
Rabbit
Positive Control
Rat kidney tissue lysates, rat testis tissue, human colon cancer tissue, human kidney tissue, human small intestine tissue, mouse brain tissue, SHSY5Y.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein affinity purified.
MOLECULAR WEIGHT
Predicted band size 75/141 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
SPATA13
SYNONYMS
APC-stimulated guanine nucleotide exchange factor 2 antibody; ARHGEF29 antibody; Asef2 antibody; FLJ31208 antibody; RP11-307N16.3 antibody; Spata13 antibody; Spermatogenesis-associated protein 13 antibody; SPT13_HUMAN antibody
TISSUE SPECIFICITY
Expressed at high levels in the placenta, spleen and kidney, at moderate levels in lung, small intestine, liver, brain and heart, and at low levels in skeletal muscle. Expression is aberrantly enhanced in most colorectal tumors.
SUBCELLULAR LOCATION
Cell membrane, Cell projection, Cytoplasm, Membrane.
FUNCTION
Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.