SPARC Recombinant Rabbit Monoclonal Antibody [PSH06-23] (HA722655)
Catalog# HA722655
Rabbit Monoclonal to SPARC
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of SPARC on different lysates with Rabbit anti-SPARC antibody (HA722655) at 1/1,000 dilution.
Lane 1: A375 cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: MG-63 cell lysate
Lane 4: RWPE-1 cell lysate
Lane 5: MCF7 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722655) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of SPARC on different lysates with Rabbit anti-SPARC antibody (HA722655) at 1/1,000 dilution.
Lane 1: A375 cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: MG-63 cell lysate
Lane 4: RWPE-1 cell lysate
Lane 5: MCF7 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722655) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.