Positive control:
Lane 1: Jurkat cell lysate
Lane 2: HepG2 cell lysate
Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
SPAK Recombinant Rabbit Monoclonal Antibody [JE53-64] (ET7110-37)
Immunogen
Synthetic peptide corresponding to c terminal human spak.
Host
Rabbit
Positive Control
Jurkat cell lysate, HepG2 cell lysate, human prostate carcinoma tissue, human pancreas tissue, Daudi.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE53-64
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
59 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB:1:500-1:2,000
-
IHC-P:1:50-1:200
-
FC:1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
SPAK
SYNONYMS
DCHT antibody; DKFZp686K05124 antibody; OTTHUMP00000165175 antibody; PASK antibody; Proline alanine rich STE20 related kinase antibody; Serine threonine kinase 39 (STE20/SPS1 homolog yeast) antibody; Serine threonine kinase 39 antibody; Serine/threonine protein kinase 39 antibody; Serine/threonine-protein kinase 39 antibody; Small intestine SPAK like kinase antibody; SPAK antibody; Ste 20 related kinase antibody; Ste-20-related kinase antibody; Ste20 like protein kinase antibody; STE20/SPS1 homolog antibody; STE20/SPS1 related proline alanine rich protein kinase antibody; STE20/SPS1-related proline-alanine-rich protein kinase antibody; STK 39 antibody; Stk39 antibody; STK39_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
TISSUE SPECIFICITY
Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated at Ser-309 by PRKCQ. Autophosphorylation at Thr-231 positively regulates its activity (By similarity).
SUBCELLULAR LOCATION
Nucleus, cytoplasm.
FUNCTION
STE20/SPS1-related proline-alanine-rich protein kinase is an enzyme that in humans is encoded by the STK39 gene. This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress. Some studies suggest that this gene might be linked to high blood pressure.