PRODUCT CODE: R1106-1

SOX2 Rabbit Polyclonal Antibody (R1106-1)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis on F9 (A) and MCF-7(B) cell lysates using anti-SOX2 rabbit polyclonal antibody.
  • Western blot analysis on F9 (A) and MCF-7(B) cell lysates using anti-SOX2 rabbit polyclonal antibody.
  • ICC image of SOX2 antibody stained human F9 cells (A) and NCCIT cells (B). The secondary antibody (green) was goat anti-rabbit IgG (H+L) FITC conjugated.
Western blot analysis on F9 (A) and MCF-7(B) cell lysates using anti-SOX2 rabbit polyclonal antibody.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

SOX2 Rabbit Polyclonal Antibody (R1106-1)

Immunogen

Peptide

Host

Rabbit

Positive Control

F9, MCF-7, NCCIT

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

33kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:20-1:50

TARGET

UNIPROT #

PROTEIN NAME

SOX2

SYNONYMS

ANOP3 antibody; cb236 antibody; Delta EF2a antibody; lcc antibody; MCOPS3 antibody; MGC148683 antibody; MGC2413 antibody; RGD1565646 antibody; Sex determining region Y box 2 antibody; SOX 2 antibody; Sox2 antibody; SOX2_HUMAN antibody; SRY (sex determining region Y) box 2 antibody; SRY box containing gene 2 antibody; SRY related HMG box 2 antibody; SRY related HMG box gene 2 antibody; SRY-box 2 antibody; Transcription factor SOX 2 antibody; Transcription factor SOX-2 antibody; ysb antibody

POST-TRANSLATIONAL MODIFICATION

Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

SRY (sex determining region Y)-box 2, also known as SOX2, is a transcription factor that is essential to maintain self-renewal of undifferentiated embryonic stem cells. Sox2, Oct-4 and Nanog co-operate in this network, and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency. POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells

CITATIONS

  • Zhu, Xinfeng et al.

    All-Trans Retinoic Acid-Induced Deficiency of the Wnt/β-Catenin Pathway Enhances Hepatic Carcinoma Stem Cell Differentiation. | PloS One [2015]