SOX2 Recombinant Rabbit Monoclonal Antibody [PO00-28] (HA721155)
Catalog# HA721155
Rabbit Monoclonal to SOX2
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IHC-P
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WB
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IF-Cell
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ChIP
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IHC-Fr
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IF-Tissue
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
Species: Mouse
Site: E14.5 embryonic brain
Sample: Frozen section
Antibody concentration: 1:1,000
Antigen retrieval: Not required
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
SOX2 Recombinant Rabbit Monoclonal Antibody [PO00-28] (HA721155)
Immunogen
Recombinant protein within human sox2 aa 1-317.
Host
Rabbit
Positive Control
NCCIT cell lysate, F9 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, NCCIT, F9, human trachea tissue, human cervical carcinoma tissue, human glioma tissue, human esophagus tissue, human tonsil tissue, human lung cancer tissue, mouse brain tissue, mouse lung tissue, rat brain tissue, rat hippocampus tissue, E14.5 mouse embryonic brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PO00-28
RRID
APPLICATION DILUTION
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IHC-P
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1:1,000-1:4,000
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WB
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1:1,000-1:2,000
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IF-Cell
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1:100-1:500
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ChIP
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Use 0.5~2 μg for 25 μg of chromatin.
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IHC-Fr
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1:1,000
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IF-Tissue
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1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 34 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
SOX2
SYNONYMS
ANOP3 antibody;cb236 antibody;Delta EF2a antibody;lcc antibody;MCOPS3 antibody;MGC148683 antibody;MGC2413 antibody;RGD1565646 antibody;Sex determining region Y box 2 antibody;SOX 2 antibody;Sox2 antibody;SOX2_HUMAN antibody;SRY (sex determining region Y) box 2 antibody;SRY box containing gene 2 antibody;SRY related HMG box 2 antibody;SRY related HMG box gene 2 antibody;SRY-box 2 antibody;Transcription factor SOX 2 antibody;Transcription factor SOX-2 antibody;ysb antibody
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
The differentiation of seminomas from non-seminomatous germ cell tumors can be challenging, especially, if small biopsy specimens, necrotic tumors and metastatic tumors with artifacts are encountered. A subset of germ cell tumors may require immunohistochemistry (IHC) for classification owing to unusual morphologic features, such as diffuse growth of clear cells, and tumors with glandular and/or microcytic patterns.1 In the mixed germ cell tumor, one component is often intermingled intimately with others such as embryonal carcinoma versus yolk sac tumor, can be overlooked. IHC will identify such an area and allow for the identification of each component of the mixed tumor more accurately and documenting them in the pathology report is recommended by WHO. Current IHC studies have shown the combination of CD30/CD117 staining plays a good role in distinguishing between embryonal carcinoma and yolk sac tumor. However, a subset of tumors may not be distinguished by this combination. Also, the characteristic membranous pattern by antibodies to CD30 and CD117 for the interpretation of the diagnosis may not be evident in limited biopsy specimens. In this respect, transcription factors, such as SOX-2, are easier to interpret due to their distinct nuclear reaction. SOX-2 has been reported as a diagnostic marker for embryonal carcinoma. SOX-2 was expressed in intratubular embryonal carcinoma, pure embryonal carcinoma and in the embryonal carcinoma component of mixed germ cell tumor in all cases. But, SOX-2 expression has not been found in seminoma, yolk sac tumor, and choriocarcinoma in almost all cases.
CITATIONS
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Liu Zhishuai,et al
Computer-assisted engineering of stable human leukemia inhibitory factor
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WB
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Human
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Citation
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