PRODUCT CODE: ET1611-89

SOX10 Recombinant Rabbit Monoclonal Antibody [SN20-41] (ET1611-89)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of SOX10 on different lysates using anti-SOX10 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Mouse brain         <br />
Lane 2: HepG2
  • Western blot analysis of SOX10 on different lysates using anti-SOX10 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Mouse brain         <br />
Lane 2: HepG2
  • ICC staining SOX10 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SOX10 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SOX10 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of SOX10 on different lysates using anti-SOX10 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse brain
Lane 2: HepG2

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

SOX10 Recombinant Rabbit Monoclonal Antibody [SN20-41] (ET1611-89)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, MCF-7, RH-35, HepG2, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN20-41

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

60 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

SOX10

SYNONYMS

DOM antibody; DOM antibody; Dominant megacolon mouse human homolog of antibody; MGC15649 antibody; PCWH antibody; SOX 10 antibody; SOX10 antibody; SOX10_HUMAN antibody; SRY (sex determining region Y) box 10 antibody; SRY (sex determining region Y) box 10 antibody; SRY box 10 antibody; SRY box containing gene 10 antibody; SRY related HMG box gene 10 antibody; SRY related HMG box gene 10 antibody; Transcription factor SOX 10 antibody; Transcription factor SOX-10 antibody; WS2E antibody; WS4 antibody; WS4C antibody

TISSUE SPECIFICITY

Expressed in fetal brain and in adult brain, heart, small intestine and colon.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.