SOCS3 Rabbit Polyclonal Antibody (ER65765)
Catalog# ER65765
Rabbit Polyclonal to SOCS3
Tested for
-
WB
-
IHC-P
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of SOCS3 on different lysates with Rabbit anti-SOCS3 antibody (ER65765) at 1/1,000 dilution.
Lane 1: PC-3M cell lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Mouse colon tissue lysate
Lane 4: Rat spleen tissue lysate
Lane 5: RAW264.7 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 30 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65765) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: PC-3M cell lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Mouse colon tissue lysate
Lane 4: Rat spleen tissue lysate
Lane 5: RAW264.7 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 30 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65765) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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