SNX27 Rabbit Polyclonal Antibody (HA500632)
Catalog# HA500632
Rabbit Polyclonal to SNX27
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of SNX27 on different lysates with Rabbit anti-SNX27 antibody (HA500632) at 1/20,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 2: 293T (Human embryonic kidney cells) cell lysate
Lane 3: U-87 MG (Human glioblastoma cells) cell lysate
Lane 4: EL4 (Mouse lymphoma cells) cell lysate
Lane 5: NIH/3T3 (Mouse fibroblasts) cell lysate
Lane 6: PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500632, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 61 kDa
Observed band size: 61 kDa
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 2: 293T (Human embryonic kidney cells) cell lysate
Lane 3: U-87 MG (Human glioblastoma cells) cell lysate
Lane 4: EL4 (Mouse lymphoma cells) cell lysate
Lane 5: NIH/3T3 (Mouse fibroblasts) cell lysate
Lane 6: PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500632, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 61 kDa
Observed band size: 61 kDa