PRODUCT CODE: ET7107-84

SNF5 Recombinant Rabbit Monoclonal Antibody [JB49-18] (ET7107-84)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of SNF5 on Daudi cell and mouse lymph node tissue lysates using anti-SNF5 antibody at 1/500 dilution.
  • Western blot analysis of SNF5 on Daudi cell and mouse lymph node tissue lysates using anti-SNF5 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SNF5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SNF5 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with SNF5 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).  Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of SNF5 on Daudi cell and mouse lymph node tissue lysates using anti-SNF5 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

SNF5 Recombinant Rabbit Monoclonal Antibody [JB49-18] (ET7107-84)

Immunogen

Synthetic peptide of c terminal human snf5.

Host

Rabbit

Positive Control

Daudi, mouse lymph node tissue lysate, rat kidney tissue, mouse colon tissue, SH-SY-5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB49-18

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

44 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

SNF5

SYNONYMS

BAF47 antibody; BRG1-associated factor 47 antibody; hSNF5 antibody; INI1 antibody; Integrase interactor 1 protein antibody; Malignant rhabdoid tumor suppressor antibody; RDT antibody; RTPS1 antibody; Sfh1p antibody; SMARCB1 antibody; SNF5 homolog antibody; SNF5_HUMAN antibody; SNF5L1 antibody; Snr1 antibody; Sucrose nonfermenting yeast homolog like 1 antibody; SWI/SNF complex component SNF5 antibody; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 antibody; SWI10 antibody; Transcription factor TYE4 antibody; Transcription regulatory protein SNF5 antibody; TYE4 antibody

SEQUENCE SIMILARITIES

Belongs to the SNF5 family.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2α) and Brg-1 (also designated SNF2β) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).