PRODUCT CODE: ER1706-90

Smad4 Rabbit Polyclonal Antibody (ER1706-90)

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

ICC staining Smad4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Smad4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Smad4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Smad4 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-Smad4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Smad4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Smad4 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HUVEC cells with Smad4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
ICC staining Smad4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Smad4 Rabbit Polyclonal Antibody (ER1706-90)

Immunogen

Synthetic peptide within human smad4 aa 500-542.

Host

Rabbit

Positive Control

A549, Hela, SH-SY5Y, rat large intestine tissue, human placenta tissue, mouse small intestine tissue, HUVEC.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

60 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Smad4

SYNONYMS

(Small) Mothers Against Decapentaplegic antibody; Deleted in Pancreatic Carcinoma 4 antibody; Deleted in Pancreatic Carcinoma antibody; Deleted in pancreatic carcinoma locus 4 antibody; Deletion target in pancreatic carcinoma 4 antibody; DPC 4 antibody; DPC4 antibody; hSMAD4 antibody; JIP antibody; MAD homolog 4 antibody; MAD mothers against decapentaplegic Drosophila homolog 4 antibody; MAD mothers against decapentaplegic homolog 4 antibody; MADH 4 antibody; MADH4 antibody; Med antibody; Medea antibody; Mothers against decapentaplegic homolog 4 antibody; Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody; Mothers against DPP homolog 4 antibody; MYHRS antibody; OTTHUMP00000163548 antibody; SMA- and MAD-related protein 4 antibody; SMAD 4 antibody; SMAD family member 4 antibody; SMAD mothers against DPP homolog 4 antibody; SMAD4 antibody; SMAD4_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the dwarfin/SMAD family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by PDPK1.; Monoubiquitinated on Lys-519 by E3 ubiquitin-protein ligase TRIM33. Monoubiquitination hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. Deubiquitination by USP9X restores its competence to mediate TGF-beta signaling.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.