PRODUCT CODE: R1510-24

SMAD3 Rabbit Polyclonal Antibody (R1510-24)

  • Zebrafish

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

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Immunocytochemical staining of Hela cells using anti-SMAD3 rabbit polyclonal antibody. .
  • Immunocytochemical staining of Hela cells using anti-SMAD3 rabbit polyclonal antibody. .
  • Immunocytochemical staining of HCT116 cells using anti-SMAD3 rabbit polyclonal antibody. .
  • Immunocytochemical staining of HepG2 cells using anti-SMAD3 rabbit polyclonal antibody. .
  • Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using anti-SMAD3 rabbit polyclonal antibody. .
  • Immunohistochemical analysis of paraffin- embedded human liver tissue using anti-SMAD3 rabbit polyclonal antibody. .
  • Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-SMAD3 rabbit polyclonal antibody. .
  • Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-SMAD3 rabbit polyclonal antibody. .
  • Immunohistochemical analysis of paraffin- embedded human stomach carcinoma tissue using anti-SMAD3 rabbit polyclonal antibody. .
  • Flow cytometric analysis of Hela cells with SMAD3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemical staining of Hela cells using anti-SMAD3 rabbit polyclonal antibody. .

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

SMAD3 Rabbit Polyclonal Antibody (R1510-24)

Immunogen

Peptide

Host

Rabbit

Positive Control

Hela, HCT116, HepG2, human colon cancer tissue, human lung carcinoma tissue, human breast carcinoma tissue, human liver tissue, human stomach carcinoma tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

48 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • WB

  • 1:500-1:1,000

TARGET

UNIPROT #

PROTEIN NAME

SMAD3

SYNONYMS

DKFZP586N0721 antibody; DKFZp686J10186 antibody; hMAD 3 antibody; hMAD-3 antibody; hSMAD3 antibody; HSPC193 antibody; HST17436 antibody; JV15 2 antibody; JV15-2 antibody; JV152 antibody; LDS1C antibody; LDS3 antibody; MAD (mothers against decapentaplegic Drosophila) homolog 3 antibody; MAD homolog 3 antibody; Mad homolog JV15 2 antibody; Mad protein homolog antibody; MAD, mothers against decapentaplegic homolog 3 antibody; Mad3 antibody; MADH 3 antibody; MADH3 antibody; MGC60396 antibody; Mothers against decapentaplegic homolog 3 antibody; Mothers against DPP homolog 3 antibody; SMA and MAD related protein 3 antibody; SMAD 3 antibody; SMAD antibody; SMAD family member 3 antibody; SMAD, mothers against DPP homolog 3 antibody; Smad3 antibody; SMAD3_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the dwarfin/SMAD family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on serine and threonine residues. Enhanced phosphorylation in the linker region on Thr-179, Ser-204 and Ser-208 on EGF and TGF-beta treatment. Ser-208 is the main site of MAPK-mediated phosphorylation. CDK-mediated phosphorylation occurs in a cell-cycle dependent manner and inhibits both the transcriptional activity and antiproliferative functions of SMAD3. This phosphorylation is inhibited by flavopiridol. Maximum phosphorylation at the G(1)/S junction. Also phosphorylated on serine residues in the C-terminal SXS motif by TGFBR1 and ACVR1. TGFBR1-mediated phosphorylation at these C-terminal sites is required for interaction with SMAD4, nuclear location and transactivational activity, and appears to be a prerequisite for the TGF-beta mediated phosphorylation in the linker region. Dephosphorylated in the C-terminal SXS motif by PPM1A. This dephosphorylation disrupts the interaction with SMAD4, promotes nuclear export and terminates TGF-beta-mediated signaling. Phosphorylation at Ser-418 by CSNK1G2/CK1 promotes ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Phosphorylated by PDPK1.; Acetylation in the nucleus by EP300 in the MH2 domain regulates positively its transcriptional activity and is enhanced by TGF-beta.; Poly-ADP-ribosylated by PARP1 and PARP2. ADP-ribosylation negatively regulates SMAD3 transcriptional responses during the course of TGF-beta signaling.; Ubiquitinated. Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. Ubiquitinated by RNF111, leading to its degradation: only SMAD3 proteins that are 'in use' are targeted by RNF111, RNF111 playing a key role in activating SMAD3 and regulating its turnover (By similarity). Undergoes STUB1-mediated ubiquitination and degradation.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, endoplasmic reticulum

FUNCTION

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.