SLC52A2 Rabbit Polyclonal Antibody (HA500611)
Catalog# HA500611
Rabbit Polyclonal to SLC52A2
Tested for
-
WB
Reacts with
-
Human
Western blot analysis of SLC52A2 on different lysates with Rabbit anti-SLC52A2 antibody (HA500611) at 1/5,000 dilution.
Lane 1: A549 cell lysate
Lane 2: A549 cell lysate (no heat)
Lane 3: HeLa cell lysate
Lane 4: HeLa cell lysate (no heat)
Lane 5: MCF7 cell lysate
Lane 6: MCF7 cell lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500611) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate
Lane 2: A549 cell lysate (no heat)
Lane 3: HeLa cell lysate
Lane 4: HeLa cell lysate (no heat)
Lane 5: MCF7 cell lysate
Lane 6: MCF7 cell lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500611) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.