SLC31A1 / CTR1 Recombinant Rabbit Monoclonal Antibody [JE56-93] (HA720005)
Catalog# HA720005
Rabbit Monoclonal to SLC31A1
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WB
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Human
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Mouse
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Rat
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: Neuro-2a cell lysate (20 µg/Lane)
Lane 3: C6 cell lysate (20 µg/Lane)
Lane 4: Mouse liver tissue lysate (40 µg/Lane)
Lane 5: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 21 kDa
Observed band size: 30 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720005) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
SLC31A1 / CTR1 Recombinant Rabbit Monoclonal Antibody [JE56-93] (HA720005)
Immunogen
Recombinant protein within human slc31a1 aa 1-82.
Host
Rabbit
Positive Control
A549 cell lysate, Neuro-2a cell lysate, C6 cell lysate, Mouse liver tissue lysate, Rat brain tissue lysate, HeLa cell lysate, Caco-2 cell lysate, C2C12 cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE56-93
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 21 kDa.
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
SLC31A1
SYNONYMS
Copper transport 1 homolog antibody;Copper transporter 1 antibody;COPT1 antibody;COPT1_HUMAN antibody;CTR1 antibody;hCTR1 antibody;High affinity copper uptake protein 1 antibody;SLC31A1 antibody;solute carrier family 31 (copper tansporters) member 1 antibody;Solute carrier family 31 member 1 antibody
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
High affinity copper uptake protein 1 (Ctr1) is a protein that in humans is encoded by the SLC31A1 gene. Copper is an element essential for life, but excessive copper can be toxic or even lethal to the cell. Therefore, cells have developed sophisticated ways to maintain a critical copper balance, with the intake, export, and intracellular compartmentalization or buffering of copper strictly regulated. The 2 related genes ATP7A (MIM 300011) and ATP7B (MIM 606882), responsible for the human diseases Menkes syndrome (MIM 309400) and Wilson disease (MIM 277900), respectively, are involved in copper export. In S. cerevisiae, the copper uptake genes CTR1, CTR2, and CTR3 have been identified, and in human the CTR1 and CTR2 (MIM 603088) genes have been identified.
CITATIONS
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A novel cuproptosis-related diagnostic gene signature and differential expression validation in atherosclerosis
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WB
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Human
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Mouse
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Citation
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