PRODUCT CODE: HA720005

SLC31A1 Recombinant Rabbit Monoclonal Antibody [JE56-93] (HA720005)

  • Recombinant

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

-
+
Western blot analysis of SLC31A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720005, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: SW480 cell lysate<br />
Lane 2: A549 cell lysate<br />
Lane 3: HepG2 cell lysate<br />
Lane 4: SKOV-3 cell lysate<br />
Lane 5: 293 cell lysate<br />
Lane 6: HCT116 cell lysate
  • Western blot analysis of SLC31A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720005, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: SW480 cell lysate<br />
Lane 2: A549 cell lysate<br />
Lane 3: HepG2 cell lysate<br />
Lane 4: SKOV-3 cell lysate<br />
Lane 5: 293 cell lysate<br />
Lane 6: HCT116 cell lysate
  • Western blot analysis of SLC31A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720005, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Rat testis tissue lysate<br />
Lane 2: Mouse spleen lysate
  • ICC staining of SLC31A1 in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720005, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of SLC31A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720005, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SW480 cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: SKOV-3 cell lysate
Lane 5: 293 cell lysate
Lane 6: HCT116 cell lysate

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

SLC31A1 Recombinant Rabbit Monoclonal Antibody [JE56-93] (HA720005)

Immunogen

Synthetic peptide within n-terminal human slc31a1.

Host

Rabbit

Positive Control

SW480 cell lysate, A549 cell lysate, HepG2 cell lysate, SKOV-3 cell lysate, 293 cell lysate, HCT116 cell lysate, rat testis tissue lysate, mouse spleen lysate, SW620.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE56-93

PROPERTIES

Form

Liquid

Storage Condition

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

Predicted band size: 21 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

SLC31A1

SYNONYMS

Copper transport 1 homolog antibody; Copper transporter 1 antibody; COPT1 antibody; COPT1_HUMAN antibody; CTR1 antibody; hCTR1 antibody; High affinity copper uptake protein 1 antibody; SLC31A1 antibody; solute carrier family 31 (copper tansporters) member 1 antibody; Solute carrier family 31 member 1 antibody

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

High affinity copper uptake protein 1 (Ctr1) is a protein that in humans is encoded by the SLC31A1 gene. Copper is an element essential for life, but excessive copper can be toxic or even lethal to the cell. Therefore, cells have developed sophisticated ways to maintain a critical copper balance, with the intake, export, and intracellular compartmentalization or buffering of copper strictly regulated. The 2 related genes ATP7A (MIM 300011) and ATP7B (MIM 606882), responsible for the human diseases Menkes syndrome (MIM 309400) and Wilson disease (MIM 277900), respectively, are involved in copper export. In S. cerevisiae, the copper uptake genes CTR1, CTR2, and CTR3 have been identified, and in human the CTR1 and CTR2 (MIM 603088) genes have been identified.