SIRT6 Rabbit Polyclonal Antibody (R1511-1)
Catalog# R1511-1
Rabbit Polyclonal to SIRT6
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WB
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IF-Cell
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FC
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Human
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Rat
Lane 1: HeLa cell lysate
Lane 2: HCT 116 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 45 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-1) at 1/4,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
SIRT6 Rabbit Polyclonal Antibody (R1511-1)
Immunogen
Recombinant protein within human sirt6 aa 1-355.
Host
Rabbit
Positive Control
HeLa cell lysate, HCT 116 cell lysate, HepG2 cell lysate, PC-12 cell lysate, HCT 116.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:4,000
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IF-Cell
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1:1,000
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 39 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
SIRT6
SYNONYMS
2810449N18Rik antibody;AI043036 antibody;Mono ADP ribosyltransferase sirtuin 6 antibody;NAD-dependent protein deacetylase sirtuin-6 antibody;Regulatory protein SIR2 homolog 6 antibody;Regulatory protein SIR2 homolog antibody;SIR2 like 6 antibody;SIR2 like protein 6 antibody;Sir2 related protein type 6 antibody;SIR2-like protein 6 antibody;SIR2L6 antibody;SIR6_HUMAN antibody;SIRT 6 antibody;SIRT6 antibody;Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody;Sirtuin 6 antibody;Sirtuin type 6 antibody;Sirtuin6 antibody
SEQUENCE SIMILARITIES
Belongs to the sirtuin family. Class IV subfamily.
SUBCELLULAR LOCATION
Nucleus, cytoplasm
FUNCTION
The Silent Information Regulator (Sir2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of this family is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging. SirT6, a mammalian homolog of Sir2, is a nuclear, chromatin-associated protein that promotes the normal maintenance of genome integrity mediated by the base excision repair (BER) pathway. The BER pathway repairs single-stranded DNA lesions that arise spontaneously from endogenous alkylation, oxidation, and deamination events. SirT6 deficient mice show increased sensitivity to DNA-damaging agents, including the alkylating agents MMS and H2O2. In addition, these mice show genome instability with increased frequency of fragmented chromosomes, detached centromeres, and gaps. SirT6 may regulate the BER pathway by deacetylating DNA Polβ or other core components of the pathway.
CITATIONS
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Sun, Y., Zheng, C., Li, T., He, X., Yang, F., Guo, W., Song, J., Gao, Y., Deng, C., & Huang, X.
GB1a Activates SIRT6 to Regulate Lipid Metabolism in Mouse Primary Hepatocytes
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WB
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IF
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Mouse
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Citation
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