SIRT6 Recombinant Rabbit Monoclonal Antibody [JJ20-69] (ET1701-29)
Catalog# ET1701-29
Rabbit Monoclonal to SIRT6
-
WB
-
Human
Lane 1: HeLa cell lysate
Lane 2: MDA-MB-231 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 2 minutes 24 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-29) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
SIRT6 Recombinant Rabbit Monoclonal Antibody [JJ20-69] (ET1701-29)
Immunogen
Synthetic peptide within human sirt6 aa 306-355 / 355.
Host
Rabbit
Positive Control
HeLa cell lysate, MDA-MB-231 cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JJ20-69
RRID
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 39 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
SIRT6
SYNONYMS
2810449N18Rik antibody;AI043036 antibody;Mono ADP ribosyltransferase sirtuin 6 antibody;NAD-dependent protein deacetylase sirtuin-6 antibody;Regulatory protein SIR2 homolog 6 antibody;Regulatory protein SIR2 homolog antibody;SIR2 like 6 antibody;SIR2 like protein 6 antibody;Sir2 related protein type 6 antibody;SIR2-like protein 6 antibody;SIR2L6 antibody;SIR6_HUMAN antibody;SIRT 6 antibody;SIRT6 antibody;Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody;Sirtuin 6 antibody;Sirtuin type 6 antibody;Sirtuin6 antibody
SEQUENCE SIMILARITIES
Belongs to the sirtuin family. Class IV subfamily.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Sirtuin 6 (SIRT6 or Sirt6) is a stress responsive protein deacetylase and mono-ADP ribosyltransferase enzyme encoded by the SIRT6 gene. In laboratory research, SIRT6 appears to function in multiple molecular pathways related to aging, including DNA repair, telomere maintenance, glycolysis and inflammation. SIRT6 is member of the mammalian sirtuin family of proteins, which are homologs to the yeast Sir2 protein. Sirt6 is mainly known as a deacetylase of histones H3 and H4, an activity by which it changes chromatin density and regulates gene expression. The enzymatic activity of Sirt6, as well as of the other members of the sirtuins family, is dependent upon the binding of the cofactor nicotinamide adenine dinucleotide (NAD+). SIRT6 is a chromatin-associated protein that is required for normal base excision repair and double-strand break repair of DNA damage in mammalian cells. SIRT6 promotes the repair of DNA double-strand breaks by the process of non-homologous end joining and homologous recombination. SIRT6 stabilizes the repair protein DNA-PKcs (DNA-dependent protein kinase catalytic subunit) at chromatin sites of damage.
CITATIONS
-
Melatonin enhances autologous adipose-derived stem cells to improve mouse ovarian function in relation to the SIRT6/NF-κB pathway
-
IHC
-
Mouse
-
Citation
-
