PRODUCT CODE: ET1701-6

SIRT5 Recombinant Rabbit Monoclonal Antibody [JJ084-01] (ET1701-6)

  • Recombinant

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

Western blot analysis of SIRT5 on Jurkat cells lysates using anti-SIRT5 antibody at 1/1,000 dilution.
  • Western blot analysis of SIRT5 on Jurkat cells lysates using anti-SIRT5 antibody at 1/1,000 dilution.
Western blot analysis of SIRT5 on Jurkat cells lysates using anti-SIRT5 antibody at 1/1,000 dilution.

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

SIRT5 Recombinant Rabbit Monoclonal Antibody [JJ084-01] (ET1701-6)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ084-01

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

37/22 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

SIRT5

SYNONYMS

NAD dependent deacetylase sirtuin 5 antibody; NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial antibody; NAD dependent protein deacylase sirtuin 5 mitochondrial antibody; NAD-dependent protein deacylase sirtuin-5, mitochondrial antibody; Regulatory protein SIR2 homolog 5 antibody; Silent mating type information regulation 2 S.cerevisiae homolog 5 antibody; Sir2 like 5 antibody; SIR2-like protein 5 antibody; SIR2L5 antibody; SIR5_HUMAN antibody; Sirt5 antibody; Sirtuin 5 antibody; Sirtuin type 5 antibody

SEQUENCE SIMILARITIES

Belongs to the sirtuin family. Class III subfamily.

TISSUE SPECIFICITY

Widely expressed.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Mitochondrion.

FUNCTION

SIRT5 is a human member of a family of proteins called Sirtuins (Sir2-like proteins) and are present in prokaryotes and eukaryotes. All Sir2-like proteins have a sirtuin core domain, which contains a series of sequence motifs conserved in organisms ranging from bacteria to humans. Bacterial, yeast and mammalian sirtuins are able to metabolize NAD and possibly at as mono-ADP-ribosyltransferases. The enzymatic function of sirtuins is not yet completely understood but recent reports of histone-activated Sir2-mediated NAD metabolism and NAD-activated Sir2-mediated histone deacetylation suggest a possible coupled reciprocal activation mechanism involving interactions of Sir2 with NAD and the N epsilon-acetyl-lysine groups of acetylated histones.