SIRT3 Rabbit Polyclonal Antibody (R1511-3)
Catalog# R1511-3
Rabbit Polyclonal to SIRT3
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WB
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Human
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Mouse
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Rat
Lane 1: PANC-1 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: Mouse liver tissue lysate (40 µg/Lane)
Lane 4: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 44 kDa
Observed band size: 28 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-3) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
SIRT3 Rabbit Polyclonal Antibody (R1511-3)
Immunogen
Recombinant protein within human sirt3 aa 51-399 / 399.
Host
Rabbit
Positive Control
PANC-1 cell lysate, HepG2 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 44 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
SIRT3
SYNONYMS
hSIRT 3 antibody;hSIRT3 antibody;Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody;NAD dependent deacetylase sirtuin 3 mitochondrial antibody;NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody;Regulatory protein SIR2 homolog 3 antibody;Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody;Sir 2 like 3 antibody;SIR 2 like protein 3 antibody;SIR 3 antibody;SIR2 L3 antibody;Sir2 like 3 antibody;SIR2 like protein 3 antibody;SIR2-like protein 3 antibody;SIR2L3 antibody;SIR3_HUMAN antibody;SIRT 3 antibody;SIRT3 antibody;Sirtuin 3 antibody;Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody;Sirtuin type 3 antibody;Sirtuin3 antibody
SEQUENCE SIMILARITIES
Belongs to the sirtuin family. Class I subfamily.
TISSUE SPECIFICITY
Widely expressed.
POST-TRANSLATIONAL MODIFICATION
Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
SUBCELLULAR LOCATION
Mitochondrion matrix
FUNCTION
The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologues, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT3 is a NAD-dependent deacetylase that contains one deacetylase sirtuin-type domain. The SIRT3 protein is widely expressed and localizes to the mitochondira where it is processed by mitochondrial processing peptidase (MPP) to yield a final product. This processing is most-likely necessary for its enzymatic activity.
CITATIONS
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Shen, M., Fan, X., Shen, Y., Wang, X., Wu, R., Wang, Y., Huang, C., Zhao, S., Zheng, Y., Men, R., Luo, X., & Yang, L.
Myeloid-derived suppressor cells ameliorate liver mitochondrial damage to protect against autoimmune hepatitis by releasing small extracellular vesicles
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IHC
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Mouse
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Citation
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Linxi Lv , Hui Jiang , Dianze Song ,et al
Sirt3 improves monosodium urate crystal-induced inflammation by suppressing Acod1 expression
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WB
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Mouse
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Citation
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Zhang Haizheng,et al
Low-level Nd:YAG laser inhibiting inflammation and oxidative stress in human gingival fibroblasts via AMPK/SIRT3 axis
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WB
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Human
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Citation
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