SIRT1 Rabbit Polyclonal Antibody (ER130811)
Catalog# ER130811
Rabbit Polyclonal to SIRT1
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WB
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IHC-P
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FC
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IF-Cell
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Human
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Mouse
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 82 kDa
Observed band size: 110 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER130811) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
SIRT1 Rabbit Polyclonal Antibody (ER130811)
Immunogen
Synthetic peptide within human sirt1 aa 698-747 / 747.
Host
Rabbit
Positive Control
Hela cell lysate, Jurkat cell lysate, F9 cell lysate, HeLa, F9, human colon carcinoma tissue, human lung carcinoma tissue, mouse liver tissue, mouse testis tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IHC-P
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1:200
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FC
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1:1,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 82 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
SIRT1
SYNONYMS
75SirT1 antibody;hSIR2 antibody;hSIRT1 antibody;HST2, S. cerevisiae, homolog of antibody;NAD dependent deacetylase sirtuin 1 antibody;NAD dependent protein deacetylase sirtuin 1 antibody;OTTHUMP00000198111 antibody;OTTHUMP00000198112 antibody;Regulatory protein SIR2 homolog 1 antibody;SIR1_HUMAN antibody;SIR2 antibody;SIR2 like 1 antibody;SIR2 like protein 1 antibody;SIR2, S.cerevisiae, homolog-like 1 antibody;SIR2-like protein 1 antibody;SIR2ALPHA antibody;SIR2L1 antibody;Sirt1 antibody;SirtT1 75 kDa fragment antibody;Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) antibody;Sirtuin 1 antibody;Sirtuin type 1 antibody
SEQUENCE SIMILARITIES
Belongs to the sirtuin family. Class I subfamily.
TISSUE SPECIFICITY
Widely expressed.
POST-TRANSLATIONAL MODIFICATION
Methylated on multiple lysine residues; methylation is enhanced after DNA damage and is dispensable for deacetylase activity toward p53/TP53.; Phosphorylated. Phosphorylated by STK4/MST1, resulting in inhibition of SIRT1-mediated p53/TP53 deacetylation. Phosphorylation by MAPK8/JNK1 at Ser-27, Ser-47, and Thr-530 leads to increased nuclear localization and enzymatic activity. Phosphorylation at Thr-530 by DYRK1A and DYRK3 activates deacetylase activity and promotes cell survival. Phosphorylation by mammalian target of rapamycin complex 1 (mTORC1) at Ser-47 inhibits deacetylation activity. Phosphorylated by CaMK2, leading to increased p53/TP53 and NF-kappa-B p65/RELA deacetylation activity (By similarity). Phosphorylation at Ser-27 implicating MAPK9 is linked to protein stability. There is some ambiguity for some phosphosites: Ser-159/Ser-162 and Thr-544/Ser-545.; Proteolytically cleaved by cathepsin B upon TNF-alpha treatment to yield catalytic inactive but stable SirtT1 75 kDa fragment (75SirT1).; S-nitrosylated by GAPDH, leading to inhibit the NAD-dependent protein deacetylase activity.; Acetylated at various Lys residues. Deacetylated via an autocatalytic mechanism. Autodeacetylation at Lys-238 promotes its protein deacetylase activity.
SUBCELLULAR LOCATION
Nucleus, cytoplasm, Mitochondrion.
FUNCTION
SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53, p300, Ku70, forkhead (FoxO) transcription factors, PPARγ, and the PPARγ coactivator-1α (PGC-1α) protein. Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival. SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol.
CITATIONS
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Luo, P., Yan, H., Chen, X., Zhang, Y., Zhao, Z., Cao, J., Zhu, Y., Du, J., Xu, Z., Zhang, X., Zeng, S., Yang, B., Ma, S., & He, Q.
s-HBEGF/SIRT1 circuit-dictated crosstalk between vascular endothelial cells and keratinocytes mediates sorafenib-induced hand-foot skin reaction that can be reversed by nicotinamide. Cell research, 30(9), 779–793.
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WB
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IF
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IHC
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Human
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Citation
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Exercise improved bone health in aging mice: a role of SIRT1 in regulating autophagy and osteogenic differentiation of BMSCs
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IF
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Mouse
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Citation
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Gong, H., Chen, H., Xiao, P., Huang, N., Han, X., Zhang, J., Yang, Y., Li, T., Zhao, T., Tai, H., Xu, W., Zhang, G., Gong, C., Yang, M., Tang, X., & Xiao, H.
miR-146a impedes the anti-aging effect of AMPK via NAMPT suppression and NAD+/SIRT inactivation
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WB
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Mouse
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Citation
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Wang, D., Geng, M., Gan, D., Han, G., Gao, G., Xing, A., Cui, Y., & Hu, Y.
Effect of resveratrol on mouse ovarian vitrification and transplantation. Reproductive biology and endocrinology : RB&E, 19(1), 54.
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WB
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IHC
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Mouse
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Citation
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