SHMT2 / SHMT Recombinant Rabbit Monoclonal Antibody [PSH19-37] (HA724069)
Catalog# HA724069
Rabbit Monoclonal to SHMT2 / SHMT
Tested for
-
WB
-
IHC-P
-
IF-Cell
-
FC
-
IP
Reacts with
-
Human
-
Mouse
-
Rat
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of SHMT2 / SHMT on different lysates with Rabbit anti-SHMT2 / SHMT antibody (HA724069) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Saos-2 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: LNCaP cell lysate
Lane 5: Jurkat cell lysate
Lane 6: COS-1 cell lysate
Lane 7: Hepa1-6 cell lysate
Lane 8: MC/9 cell lysate
Lane 9: Mouse liver tissue lysate
Lane 10: Mouse kidney tissue lysate
Lane 11: Mouse skeletal muscle tissue lysate (low expression)
Lane 12: Rat liver tissue lysate
Lane 13: Rat kidney tissue lysate
Lane 14: Rat skeletal muscle tissue lysate (low expression)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 3 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724069) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of SHMT2 / SHMT on different lysates with Rabbit anti-SHMT2 / SHMT antibody (HA724069) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Saos-2 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: LNCaP cell lysate
Lane 5: Jurkat cell lysate
Lane 6: COS-1 cell lysate
Lane 7: Hepa1-6 cell lysate
Lane 8: MC/9 cell lysate
Lane 9: Mouse liver tissue lysate
Lane 10: Mouse kidney tissue lysate
Lane 11: Mouse skeletal muscle tissue lysate (low expression)
Lane 12: Rat liver tissue lysate
Lane 13: Rat kidney tissue lysate
Lane 14: Rat skeletal muscle tissue lysate (low expression)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 3 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724069) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.