PRODUCT CODE: ET7109-27

SFRP4 Recombinant Rabbit Monoclonal Antibody [JE41-41] (ET7109-27)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of SFRP4 on different lysates using anti-SFRP4 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse testis <br />
    Lane 2: A549<br />
 Lane 3: HepG2 <br />
           Lane 4: SH-SY-5Y
  • Western blot analysis of SFRP4 on different lysates using anti-SFRP4 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse testis <br />
    Lane 2: A549<br />
 Lane 3: HepG2 <br />
           Lane 4: SH-SY-5Y
  • Flow cytometric analysis of A549 cells with SFRP4 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).  Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of SFRP4 on different lysates using anti-SFRP4 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse testis
Lane 2: A549
Lane 3: HepG2
Lane 4: SH-SY-5Y

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

SFRP4 Recombinant Rabbit Monoclonal Antibody [JE41-41] (ET7109-27)

Immunogen

Full length recombinant protein of human sfrp4.

Host

Rabbit

Positive Control

Mouse testis tissue, A549, HepG2, SH-SY-5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE41-41

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

40 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

SFRP4

SYNONYMS

Frizzled protein antibody; Frizzled protein human endometrium antibody; FRP 4 antibody; FRP4 antibody; FrpHE antibody; human endometrium antibody; PYL antibody; Secreted frizzled-related protein 4 antibody; sFRP-4 antibody; Sfrp4 antibody; SFRP4_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the secreted frizzled-related protein (sFRP) family.

TISSUE SPECIFICITY

Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level).

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 plays a role in bone morphogenesis. May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.