SFPQ Recombinant Rabbit Monoclonal Antibody [JU33-99] (ET7107-02)

UNIPROT #

P23246

PROTEIN NAME

SFPQ

SYNONYMS

100 kDa DNA pairing protein antibody;100 kDa DNA-pairing protein antibody;100 kDa subunit antibody;DNA binding p52/p100 complex 100 kDa subunit antibody;DNA-binding p52/p100 complex antibody;hPOMp100 antibody;Polypyrimidine tract binding protein associated splicing factor antibody;Polypyrimidine tract-binding protein-associated-splicing factor antibody;POMP100 antibody;PPP1R140 antibody;proline- and glutamine-rich antibody;Protein phosphatase 1 regulatory subunit 140 antibody;PSF antibody;PTB associated splicing factor antibody;PTB-associated-splicing factor antibody;Sfpq antibody;SFPQ_HUMAN antibody;Splicing factor antibody;Splicing factor proline and glutamine rich antibody;Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) antibody;Splicing factor proline/glutamine rich antibody

POST-TRANSLATIONAL MODIFICATION

The N-terminus is blocked.; Phosphorylated on multiple serine and threonine residues during apoptosis. In vitro phosphorylated by PKC. Phosphorylation stimulates binding to DNA and D-loop formation, but inhibits binding to RNA. Phosphorylation of C-terminal tyrosines promotes its cytoplasmic localization, impaired its binding to polypyrimidine RNA and led to cell cycle arrest. In resting T-cells is phosphorylated at Thr-687 by GSK3B which is proposed to promote association with THRAP and to prevent binding to PTPRC/CD45 pre-mRNA; T-cell activation leads to reduced phosphorylation at Thr-687.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation.