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sfGFP Rabbit Polyclonal Antibody (ER1802-92)

Price: Regular price $315.00

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Catalog# ER1802-92

Rabbit Polyclonal to sfGFP

Tested for

  • WB

Reacts with

  • Species independent

Specifications

Product Type

Rabbit polyclonal primary

Product Name

sfGFP Rabbit Polyclonal Antibody (ER1802-92)

Immunogen

Synthetic peptide immune sequence is rdhmvlheyvnaagitc.

Host

Rabbit

Positive Control

GFP11-tagged fusion protein.

Conjugation

Unconjugated

Clonality

Polyclonal

RRID

AB_3069192

APPLICATION DILUTION

  • WB

  • 1:1,000

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Immunogen affinity purified.

Isotype

IgG

TARGET

PROTEIN NAME

sfGFP

FUNCTION

Tagging proteins with a functional sequence is a powerful way to access function, and inserting tags at endogenous genomic loci allows the preservation of a near-native cellular background. GFP11 is the 11th beta-strand of the superfolder GFP beta-barrel structure. When expressed in the same cell, GFP11 and its complementary GFP fragment (GFP1-10) enable functional GFP tagging upon complementation. The key advantage of the GFP11 is its small size (16 aa): this allows commercial ssDNA oligomers to be used as HDR donors, circumventing any requirement for molecular cloning.

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