Applications
-
WB
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
sfGFP Rabbit Polyclonal Antibody (ER1802-92)
Immunogen
Synthetic peptide immune sequence is rdhmvlheyvnaagitc.
Host
Rabbit
Positive Control
GFP11-tagged fusion protein.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Immunogen affinity purified.
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000
TARGET
PROTEIN NAME
Anti-sfGFP antibdody
FUNCTION
Tagging proteins with a functional sequence is a powerful way to access function, and inserting tags at endogenous genomic loci allows the preservation of a near-native cellular background. GFP11 is the 11th beta-strand of the superfolder GFP beta-barrel structure. When expressed in the same cell, GFP11 and its complementary GFP fragment (GFP1-10) enable functional GFP tagging upon complementation. The key advantage of the GFP11 is its small size (16 aa): this allows commercial ssDNA oligomers to be used as HDR donors, circumventing any requirement for molecular cloning.