PRODUCT CODE: ER1803-06

SERPINC1 Rabbit Polyclonal Antibody (ER1803-06)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of SERPINC1 on different lysates using anti-SERPINC1 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: HepG2   <br />
    Lane 2: Mouse smooth muscle<br />
 Lane 3: Mouse lung
  • Western blot analysis of SERPINC1 on different lysates using anti-SERPINC1 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: HepG2   <br />
    Lane 2: Mouse smooth muscle<br />
 Lane 3: Mouse lung
  • ICC staining SERPINC1 in H22 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SERPINC1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SERPINC1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SERPINC1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-SERPINC1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with SERPINC1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of SERPINC1 on different lysates using anti-SERPINC1 antibody at 1/500 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Mouse smooth muscle
Lane 3: Mouse lung

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

SERPINC1 Rabbit Polyclonal Antibody (ER1803-06)

Immunogen

Recombinant protein within human serpinc1 aa 150-400.

Host

Rabbit

Positive Control

HepG2, mouse smooth muscle tissue, mouse lung tissue, H22, rat kidney tissue, human liver tissue, human small intestine tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

53 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1

  • 1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

SERPINC1

SYNONYMS

ANT3_HUMAN antibody; Antithrombin antibody; Antithrombin III antibody; Antithrombin-III antibody; AntithrombinIII antibody; AT 3 antibody; AT III antibody; AT3 antibody; AT3D antibody; ATIII antibody; Heparin cofactor I antibody; MGC22579 antibody; Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 antibody; Serine cysteine proteinase inhibitor clade C member 1 antibody; Serine proteinase inhibitor clade C member 1 antibody; Serpin C1 antibody; Serpin family C member 1 antibody; Serpin peptidase inhibitor clade C (antithrombin) member 1 antibody; SERPINC1 antibody; THPH7 antibody

SEQUENCE SIMILARITIES

Belongs to the serpin family.

TISSUE SPECIFICITY

Found in plasma.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by FAM20C in the extracellular medium.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

The serine proteinase inhibitors (serpins) compose a superfamily of proteins with a diverse set of functions, including the control of blood coagulation, complement activation, programmed cell death and development. Serpins are secreted glycoproteins that contain a stretch of peptide that mimics a true substrate for a corresponding serine protease. Antithrombin III (ATIII), an extracellular plasma protein, is a crucial serine protease inhibitor that regulates the coagulation cascade in blood. The inhibitory activity of ATIII is amplified in the presence of heparin. ATIII inhibits Thrombin and Factors IXa, Xa and XIa. Defects in the gene SERPINC1, which encodes for ATIII, can cause ATIII deficiency, an autosomal dominant disease which is a risk factor for hereditary thrombophilia.