PRODUCT CODE: EM0704

Sequestosome-1 Mouse Monoclonal Antibody [10-E10] (EM0704)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of SQSTM1 on different cell lysates using anti-SQSTM1 antibody at 1/500 dilution.<br />
Positive control: <br />
Lane 1: Hela<br />
Lane 2: HepG2
  • Western blot analysis of SQSTM1 on different cell lysates using anti-SQSTM1 antibody at 1/500 dilution.<br />
Positive control: <br />
Lane 1: Hela<br />
Lane 2: HepG2
  • ICC staining SQSTM1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti- SQSTM1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti- SQSTM1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-SQSTM1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with SQSTM1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
Western blot analysis of SQSTM1 on different cell lysates using anti-SQSTM1 antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Sequestosome-1 Mouse Monoclonal Antibody [10-E10] (EM0704)

Immunogen

Synthetic peptide within human sequestosome-1 aa 42-91 / 440.

Host

Mouse

Positive Control

Hela, HepG2, mouse spleen, mouse prostate, mouse pancreas

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

10-E10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

62 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1 ?g per 1 x 106 cells

TARGET

UNIPROT #

PROTEIN NAME

Sequestosome-1

SYNONYMS

A170 antibody; DMRV antibody; EBI 3 associated protein of 60 kDa antibody; EBI 3 associated protein p60 antibody; EBI3 associated protein of 60 kDa antibody; EBI3 associated protein p60 antibody; EBI3-associated protein of 60 kDa antibody; EBIAP antibody; FTDALS3 antibody; MGC127197 antibody; ORCA antibody; OSF-6 antibody; Osi antibody; OSIL antibody; Oxidative stress induced like antibody; p60 antibody; p62 antibody; p62B antibody; Paget disease of bone 3 antibody; PDB 3 antibody; PDB3 antibody; Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody; Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody; PKC-zeta-interacting protein antibody; Protein kinase C-zeta-interacting protein antibody; Sequestosome 1 antibody; Sequestosome-1 antibody; SQSTM 1 antibody; SQSTM_HUMAN antibody; Sqstm1 antibody; STAP antibody; STONE14 antibody; Ubiquitin binding protein p62 antibody; Ubiquitin-binding protein p62 antibody; ZIP 3 antibody; ZIP antibody; ZIP3 antibody

TISSUE SPECIFICITY

Ubiquitously expressed.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN. Phosphorylation at Ser-403 by ULK1 is stimulated by SESN2. Phosphorylated at Ser-403 by TBK1, leading to promote relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes. Phosphorylation at Ser-349 by MTOR promotes interaction with KEAP1 and inactivation of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes (By similarity).; Ubiquitinated by RNF26: ubiquitinated SQSTM1 attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport. Deubiquitination by USP15 releases target vesicles for fast transport into the cell periphery. Ubiquitinated by the BCR(KEAP1) complex at Lys-420, increasing SQSTM1 sequestering activity and promoting its degradation. Ubiquitinated via 'Lys-29' and 'Lys-33'-linked polyubiquitination leading to xenophagic targeting of bacteria and inhibition of their replication.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus

FUNCTION

Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress and autophagy. It was first identified as a protein that binds to the SH2 domain of p56Lck and independently found to interact with PKCζ. SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome. Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway. It may play a role in titin/TTN downstream signaling in muscle cells and regulate signaling cascades through ubiquitination. P62 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels.

CITATIONS

  • Zhang, Wenkan et al.

    Water Extract of Sporoderm-Broken Spores of Ganoderma lucidum Induces Osteosarcoma Apoptosis and Restricts Autophagic Flux. | OncoTargets and Therapy [2019]

  • Zhang, D. G., Zhao, T., Hog...

    Oxidized fish oils increased lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the CREB1-Bcl2-Beclin1 pathway in the liver tissues and hepatocytes of yellow catfish. Food chemistry, 360, 129814.

  • Chen, Lili et al.

    Autophagy negative-regulating Wnt signaling enhanced inflammatory osteoclastogenesis from Pre-OCs in vitro. | Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie [2020]