PRODUCT CODE: ET7108-53

SENP1 Recombinant Rabbit Monoclonal Antibody [JG37-79] (ET7108-53)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

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Western blot analysis of SENP1 on K562 cell using anti-SENP1 antibody at 1/1,000 dilution.
  • Western blot analysis of SENP1 on K562 cell using anti-SENP1 antibody at 1/1,000 dilution.
  • ICC staining SENP1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SENP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SENP1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SENP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human small Intestine tissue using anti-SENP1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Daudi cells with SENP1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).  Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of SENP1 on K562 cell using anti-SENP1 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

SENP1 Recombinant Rabbit Monoclonal Antibody [JG37-79] (ET7108-53)

Immunogen

Recombinant protein within human senp1 aa 300-500.

Host

Rabbit

Positive Control

K562, LOVO, SH-SY-5Y, SiHa, Daudi, human liver tissue, human small Intestine tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG37-79

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

73 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

SENP1

SYNONYMS

SENP 1 antibody; SENP1 antibody; SENP1_HUMAN antibody; Sentrin specific protease 1 antibody; Sentrin-specific protease 1 antibody; Sentrin/SUMO specific protease 1 antibody; Sentrin/SUMO specific protease antibody; Sentrin/SUMO specific protease SENP 1 antibody; Sentrin/SUMO specific protease SENP1 antibody; Sentrin/SUMO-specific protease SENP1 antibody; SUMO1/sentrin specific peptidase 1 antibody; SUMO1/sentrin specific protease 1 antibody; SuPr 2 antibody; SuPr2 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C48 family.

TISSUE SPECIFICITY

Highly expressed in testis. Expressed at lower levels in thymus, pancreas, spleen, liver, ovary and small intestine.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Protease that catalyzes two essential functions in the SUMO pathway. The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1 and BHLHE40/DEC1, which decreases its transcriptional repression activity. Deconjugates SUMO1 from CLOCK, which decreases its transcriptional activation activity. Deconjugates SUMO2 from MTA1. Desumoylates CCAR2 which decreases its interaction with SIRT1. Deconjugates SUMO1 from GPS2.