SEC62 Recombinant Rabbit Monoclonal Antibody [PSH0-25] (HA721305)
Catalog# HA721305
Rabbit Monoclonal to SEC62
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (HA721305) at 1/5,000 dilution.
Lane 1: Hela cell lysate
Lane 2: Hela cell lysate
Lane 3: K562 cell lysate
Lane 4: SH-SY5Y cell lysate
Lane 5: HepG2 cell lysate
Lane 6: 293T cell lysate
Lane 7: Jurkat cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 56 kDa
Exposure time: 30 seconds;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721305) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Lane 1: Hela cell lysate
Lane 2: Hela cell lysate
Lane 3: K562 cell lysate
Lane 4: SH-SY5Y cell lysate
Lane 5: HepG2 cell lysate
Lane 6: 293T cell lysate
Lane 7: Jurkat cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 56 kDa
Exposure time: 30 seconds;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721305) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.