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PRODUCT CODE: ER1803-63

SDHB Rabbit Polyclonal Antibody (ER1803-63)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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All lanes: Western blot analysis of SDHB with anti-SDHB antibody (ER1803-63) at 1:1,000 dilution.<br /> Lane 1: Wild-type Hela whole cell lysate.<br /> Lane 2: SDHB knockout Hela whole cell lysate.<br /> <br /> ER1803-63 was shown to specifically react with SDHB in wild-type Hela cells. No band was observed when SDHB knockout samples were tested. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-SDHB antibody (ER1803-63, 1/1,000) and Anti-GAPDH antibody (ET1601-4, 1/10000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • All lanes: Western blot analysis of SDHB with anti-SDHB antibody (ER1803-63) at 1:1,000 dilution.<br /> Lane 1: Wild-type Hela whole cell lysate.<br /> Lane 2: SDHB knockout Hela whole cell lysate.<br /> <br /> ER1803-63 was shown to specifically react with SDHB in wild-type Hela cells. No band was observed when SDHB knockout samples were tested. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-SDHB antibody (ER1803-63, 1/1,000) and Anti-GAPDH antibody (ET1601-4, 1/10000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of SDHB on different cell lysate using anti-SDHB antibody at 1/1,000 dilution.<br /> Positive control:<br /> Lane 1: human liver<br /> Lane 2: mouse liver<br /> Lane 3: rat liver<br /> Lane 4: rat liver, preincubated with the immunization protein.
  • ICC staining SDHB in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SDHB in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-SDHB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SDHB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-SDHB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SDHB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SDHB antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293T cells with SDHB antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
All lanes: Western blot analysis of SDHB with anti-SDHB antibody (ER1803-63) at 1:1,000 dilution.
Lane 1: Wild-type Hela whole cell lysate.
Lane 2: SDHB knockout Hela whole cell lysate.

ER1803-63 was shown to specifically react with SDHB in wild-type Hela cells. No band was observed when SDHB knockout samples were tested. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-SDHB antibody (ER1803-63, 1/1,000) and Anti-GAPDH antibody (ET1601-4, 1/10000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

SDHB Rabbit Polyclonal Antibody (ER1803-63)

Immunogen

Recombinant protein within human sdhb aa 100-280.

Host

Rabbit

Positive Control

Rat liver tissue, human liver tissue, mouse liver tissue, 293T, HT-29, Hela, rat heart tissue, human kidney tissue, human skin tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

32 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P21912

PROTEIN NAME

SDHB

SYNONYMS

CWS2 antibody; DHSB_HUMAN antibody; FLJ92337 antibody; Ip antibody; Iron sulfur subunit antibody; Iron sulfur subunit of complex II antibody; Iron-sulfur subunit of complex II antibody; mitochondrial antibody; PGL 4 antibody; PGL4 antibody; SDH 1 antibody; SDH antibody; SDH1 antibody; SDH2 antibody; SDH2, homolog of antibody; SdhB antibody; SDHIP antibody; Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial antibody; Succinate dehydrogenase [ubiquinone] iron sulfur subunit antibody; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit antibody; succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial antibody; Succinate Dehydrogenase 1 Iron Sulfur Subunit antibody; Succinate Dehydrogenase 2, S. cerevisiae, homolog of antibody; Succinate dehydrogenase complex iron sulfur subunit B antibody; Succinate dehydrogenase complex subunit B iron sulfur antibody; Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein antibody; succinate dehydrogenase complex, subunit B, iron sulfur (Ip) antibody; Succinate dehydrogenase iron sulfur protein antibody

SEQUENCE SIMILARITIES

Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.

SUBCELLULAR LOCATION

Mitochondrion.

FUNCTION

In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations associate with juvenile encephalopathy as well as Leigh syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma.