SDHB Recombinant Rabbit Monoclonal Antibody [JU02-33] (ET1706-30)
Catalog# ET1706-30
Rabbit Monoclonal to SDHB
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WB
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IHC-P
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Human
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Mouse
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Rat
All lanes: Western blot analysis of SDHB with anti-SDHB antibody [JU02-33] (ET1706-30) at 1:1,000 dilution.
Lane 1: Wild-type Hela whole cell lysate.
Lane 2: SDHB knockout Hela whole cell lysate.
ET1706-30 was shown to specifically react with SDHB in wild-type Hela cells. No band was observed when SDHB knockout samples were tested. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-SDHB antibody (ET1706-30, 1/1,000) and Anti-GAPDH antibody (ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
SDHB Recombinant Rabbit Monoclonal Antibody [JU02-33] (ET1706-30)
Immunogen
Synthetic peptide within human sdhb aa 201-250 / 280.
Host
Rabbit
Positive Control
HepG2 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, Rat liver tissue lysate, Human liver tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, human liver tissue, human colon carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JU02-33
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IHC-P
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 32 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
SDHB
SYNONYMS
CWS2 antibody;DHSB_HUMAN antibody;FLJ92337 antibody Ip antibody;Iron sulfur subunit antibody;Iron sulfur subunit of complex II antibody;Iron-sulfur subunit of complex II antibody;mitochondrial antibody;PGL 4 antibody;PGL4 antibody;SDH 1 antibody;SDH antibody;SDH1 antibody;SDH2 antibody;SDH2, homolog of antibody;SdhB antibody;SDHIP antibody;Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial antibody;Succinate dehydrogenase [ubiquinone] iron sulfur subunit antibody;Succinate dehydrogenase [ubiquinone] iron-sulfur subunit antibody;succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial antibody;Succinate Dehydrogenase 1 Iron Sulfur Subunit antibody;Succinate Dehydrogenase 2, S. cerevisiae, homolog of antibody;Succinate dehydrogenase complex iron sulfur subunit B antibody;Succinate dehydrogenase complex subunit B iron sulfur antibody;Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein antibody;succinate dehydrogenase complex, subunit B, iron sulfur (Ip) antibody;Succinate dehydrogenase iron sulfur protein antibody
SEQUENCE SIMILARITIES
Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.
SUBCELLULAR LOCATION
Mitochondrion. Mitochondrion inner membrane.
FUNCTION
In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations associate with juvenile encephalopathy as well as Leigh syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma.
CITATIONS
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Knockdown of PGC1α suppresses dysplastic oral keratinocytes proliferation through reprogramming energy metabolism
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WB
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IHC
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Human
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Citation
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