SDHB Recombinant Rabbit Monoclonal Antibody [JU02-33] (ET1706-30)
Catalog# ET1706-30
Rabbit Monoclonal to SDHB
Tested for
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WB
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IHC-P
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FC
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IP
React with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
All lanes: Western blot analysis of SDHB with anti-SDHB antibody [JU02-33] (ET1706-30) at 1:1,000 dilution.
Lane 1: Wild-type Hela whole cell lysate.
Lane 2: SDHB knockout Hela whole cell lysate.
ET1706-30 was shown to specifically react with SDHB in wild-type Hela cells. No band was observed when SDHB knockout samples were tested. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-SDHB antibody (ET1706-30, 1/1,000) and Anti-GAPDH antibody (ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
Lane 1: Wild-type Hela whole cell lysate.
Lane 2: SDHB knockout Hela whole cell lysate.
ET1706-30 was shown to specifically react with SDHB in wild-type Hela cells. No band was observed when SDHB knockout samples were tested. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-SDHB antibody (ET1706-30, 1/1,000) and Anti-GAPDH antibody (ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
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