SCA-1 Rabbit Polyclonal Antibody (0804-10)

Western blot analysis on Mouse liver using anti-SCA-1 polyclonal antibody

Applications

REACTIVITY

Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

SCA-1 Rabbit Polyclonal Antibody (0804-10)

Immunogen

Synthetic peptide within mouse sca-1 aa 51-94.

Host

Rabbit

Positive Control

Mouse liver

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

18-20kDa

Isotype

IgG

APPLICATION DILUTION

WB
1:1,000

TARGET

UNIPROT #

P05533

PROTEIN NAME

SCA-1

SYNONYMS

Ly-6A.2/Ly-6E. antibody; Ly-6A.2/Ly-6E.1 antibody; Ly6a antibody; LY6A_MOUSE antibody; Ly6al antibody; Lymphocyte antigen 6A-2/6E-1 antibody; Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody; SCA-1 antibody; Stem cell antigen 1 antibody; T cell activating protein antibody; T-cell-activating protein antibody; TAP antibody

TISSUE SPECIFICITY

Widely expressed.

POST-TRANSLATIONAL MODIFICATION

O-glycosylated. Not N-glycosylated.; Not phosphorylated.

SUBCELLULAR LOCATION

Cell membrane

FUNCTION

Sca-1, an 18 kDa phosphatidylinositol-anchored protein, is a member of the Ly-6 antigen family. Sca-1 is the most recognized HSC marker in mice with both Ly-6 haplotypes as it is expressed on multipotent HSCs. Sca-1+ HSCs can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen within the adult animal. An anti-Sca-1 Ab is frequently used in combination with negative selection for expression of a number of cell surface markers characteristic of differentiated cells of hematolymphoid lineages (Lin-) to identify and isolate murine HSCs.