SCA-1 Rabbit Polyclonal Antibody (0804-10)
Catalog# 0804-10
Rabbit Polyclonal to SCA-1
Tested for
-
WB
-
IF-Cell
React with
-
Mouse
Western blot analysis of SCA-1 on different lysates with Rabbit anti-SCA-1 antibody (0804-10) at 1/500 dilution.
Lane 1: Mouse kidney tissue lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (10 µg/Lane)
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0804-10) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse kidney tissue lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (10 µg/Lane)
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0804-10) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
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