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PRODUCT CODE: ER1902-38

SARS2 Rabbit Polyclonal Antibody (ER1902-38)

  • Zebrafish

Applications

  • WB

  • IF

  • IHC-P

REACTIVITY

  • Zebrafish

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Western blot analysis of SARS2 on Zebrafish tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of SARS2 on Zebrafish tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • IF staining of SARS2 in Zebrafish (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-SARS2 antibody. Counter stained with hematoxylin.
Western blot analysis of SARS2 on Zebrafish tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IF

  • IHC-P

REACTIVITY

  • Zebrafish

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

SARS2 Rabbit Polyclonal Antibody (ER1902-38)

Immunogen

Recombinant protein within zebrafish sars2 aa 289-510 / 510.

Host

Rabbit

Positive Control

Zebrafish.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

58 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

A3QJX4

PROTEIN NAME

SARS2

SYNONYMS

mitochondrial antibody; mtSerRS antibody; SARS antibody; SARS2 antibody; SARSM antibody; Serine tRNA ligase antibody; Serine--tRNA ligase antibody; SerRS antibody; SerRSmt antibody; SERS antibody; Seryl tRNA synthetase 2 antibody; Seryl-tRNA synthetase antibody; Seryl-tRNA(Ser/Sec) synthetase antibody; SYS antibody; SYSM_HUMAN antibody

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Involved in ATP binding, has serine-tRNA ligase activity and involved in seryl-tRNA aminoacylation.