Applications
-
ELISA
-
WB
REACTIVITY
-
Sars-cov-2
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
SARS-CoV-2 Nucleocapsid Protein Rabbit Polyclonal Antibody (ER1902-39)
Immunogen
Full length of sars-cov-2 nucleocapsid protein.
Host
Rabbit
Positive Control
Recombinant SARS-CoV-2 Nucleocapsid Protein.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Antigen affinity purified.
MOLECULAR WEIGHT
48 kDa
Isotype
IgG
APPLICATION DILUTION
-
ELISA
-
1:1,000-1:5,000
-
WB:1:500-1:2,000
TARGET
UNIPROT #
PROTEIN NAME
SARS-CoV-2 Nucleocapsid Protein
SYNONYMS
SARS-CoV-2 Nucleocapsid Protein antibody; SARS-CoV-2 NP antibody; nucleocapsid protein antibody; novel coronavirus N Protein antibody; novel coronavirus Nucleocapsid Protein antibody; 2019-nCoV Nucleoprotein antibody; 2019-nCoV N antibody; 2019nCoV N antibody; 2019-nCoV N Protein antibody; 2019 peak N Protein antibody; 2019-nCoV nucleocapsid protein antibody
SUBCELLULAR LOCATION
Cytoplasm of SARS-CoV-2.
FUNCTION
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.