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PRODUCT CODE: ER1902-39

SARS-CoV-2 Nucleocapsid Protein Rabbit Polyclonal Antibody (ER1902-39)

Applications

  • ELISA

  • WB

REACTIVITY

  • SARS-CoV-2

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ELISA analysis with anti-SARS-CoV-2 Nucleocapsid Proein antibody. Antigen (1 μg/mL). The antigen was used as the coating antigen, and the anti-SARS-CoV-2 Nucleocapsid Protein antibody was used as the capture antigen for ELISA.
  • ELISA analysis with anti-SARS-CoV-2 Nucleocapsid Proein antibody. Antigen (1 μg/mL). The antigen was used as the coating antigen, and the anti-SARS-CoV-2 Nucleocapsid Protein antibody was used as the capture antigen for ELISA.
  • Western blot using anti-SARS-CoV-2 Nucleocapsid Protein antibody shows detection of a 48 kDa band corresponding to the protein.
ELISA analysis with anti-SARS-CoV-2 Nucleocapsid Proein antibody. Antigen (1 μg/mL). The antigen was used as the coating antigen, and the anti-SARS-CoV-2 Nucleocapsid Protein antibody was used as the capture antigen for ELISA.

Applications

  • ELISA

  • WB

REACTIVITY

  • SARS-CoV-2

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

SARS-CoV-2 Nucleocapsid Protein Rabbit Polyclonal Antibody (ER1902-39)

Immunogen

Full length of sars-cov-2 nucleocapsid protein.

Host

Rabbit

Positive Control

Recombinant SARS-CoV-2 Nucleocapsid Protein.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Antigen affinity purified.

MOLECULAR WEIGHT

48 kDa

Isotype

IgG

APPLICATION DILUTION

  • ELISA

  • 1:1,000-1:5,000

  • WB:1:500-1:2,000

TARGET

UNIPROT #

P0DTC9

PROTEIN NAME

SARS-CoV-2 Nucleocapsid Protein

SYNONYMS

SARS-CoV-2 Nucleocapsid Protein antibody; SARS-CoV-2 NP antibody; nucleocapsid protein antibody; novel coronavirus N Protein antibody; novel coronavirus Nucleocapsid Protein antibody; 2019-nCoV Nucleoprotein antibody; 2019-nCoV N antibody; 2019nCoV N antibody; 2019-nCoV N Protein antibody; 2019 peak N Protein antibody; 2019-nCoV nucleocapsid protein antibody

SUBCELLULAR LOCATION

Cytoplasm of SARS-CoV-2.

FUNCTION

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.