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Western blot analysis of SAMHD1 on THP-1 cell lysates with Rabbit anti-SAMHD1 antibody (ET1706-24) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Exposure time: 30 seconds;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-24) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
CHBL2 antibody;DCIP antibody;Dendritic cell derived IFNG induced protein antibody;Dendritic cell-derived IFNG-induced protein antibody;Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 antibody;dNTPase antibody;HD domain containing 1 antibody;HDDC1 antibody;Mg11 antibody;Monocyte protein 5 antibody;MOP 5 antibody;MOP-5 antibody;MOP5 antibody;OTTHUMP00000030889 antibody;SAM domain and HD domain 1 antibody;SAM domain and HD domain containing protein 1 antibody;SAM domain and HD domain-containing protein 1 antibody;SAMH1_HUMAN antibody;Samhd1 antibody;SBBI88 antibody
Belongs to the SAMHD1 family.
Expressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung and peripheral blood leukocytes. No expression is seen in brain and thymus.
Phosphorylation at Thr-592 by CDK1 acts as a switch to control deoxynucleoside triphosphate (dNTPase)-dependent and -independent functions. Phosphorylation at Thr-592 takes place in cycling cells: it reduces the stability of the homotetramer, impairing the dNTPase activity and subsequent ability to restrict infection by viruses. It also inhibits ability to suppress LINE-1 retrotransposon activity. In contrast, phosphorylation at Thr-592 promotes DNA end resection at stalled replication forks in response to DNA damage.; (Microbial infection) Ubiquitinated following interaction with HIV-2 viral protein Vpx; Vpx promotes interaction and with a DCX (DDB1-CUL4-X-box) E3 ubiquitin ligase, leading to proteasomal degradation.
SAM domain and HD domain-containing protein 1 is a protein that in humans is encoded by the SAMHD1 gene. SAMHD1 is a cellular enzyme, responsible for blocking replication of HIV in dendritic cells, macrophages and monocytes. It is an enzyme that exhibits phosphohydrolase activity, converting deoxynucleoside triphosphates (dNTPs) to inorganic phosphate (iPPP) and a 2'-deoxynucleoside (i.e. deoxynucleosides without a phosphate group). In doing so, SAMHD1 depletes the pool of dNTPs available to a reverse transcriptase for viral cDNA synthesis and thus prevents viral replication. SAMHD1 has also shown nuclease activity. Although a ribonuclease activity was described to be required for HIV-1 restriction, recent data confirmed that SAMHD1-mediated HIV-1 restriction in cells does not involve ribonuclease activity.
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.