SALL4 Mouse Monoclonal Antibody [A9G10] (HA601145)
Catalog# HA601145
Mouse Monoclonal to SALL4
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IHC-P
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WB
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FC
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IF-Cell
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IF-Tissue
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Human
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601145) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Specifications
Product Type
Mouse monoclonal primary
Product Name
SALL4 Mouse Monoclonal Antibody [A9G10] (HA601145)
Immunogen
Recombinant protein within human sall4 aa 904-1,053/1,053.
Host
Mouse
Positive Control
Human seminoma tissue, human testis tissue, NCCIT cell lysates, NCCIT.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A9G10
RRID
APPLICATION DILUTION
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IHC-P
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1:4,000
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WB
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1:1,000
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FC
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1:500-1:1,000
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IF-Cell
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1:3,000
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IF-Tissue
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1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 112 kDa
Isotype
IgG2b
TARGET
UNIPROT #
PROTEIN NAME
SALL4
SYNONYMS
AA407717 antibody;AL022809 antibody;AW536104 antibody;C330011P20Rik antibody;C78083 antibody;C78563 antibody;dJ1112F19.1 antibody;DRRS antibody;HSAL4 antibody;Sal like 4 (Drosophila) antibody;Sal like 4 antibody;Sal like Protein 4 antibody;Sal-like protein 4 antibody;Sall4 antibody;SALL4_HUMAN antibody;Spalt like transcription factor 4 antibody;Tex20 antibody;Zinc finger protein 797 antibody;Zinc finger protein SALL4 antibody;ZNF797 antibody
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Sal-like protein 4 (SALL4) is a transcription factor encoded by a member of the Spalt-like (SALL) gene family, SALL4. The SALL genes were identified based on their sequence homology to Spalt, which is a homeotic gene originally cloned in Drosophila melanogaster that is important for terminal trunk structure formation in embryogenesis and imaginal disc development in the larval stages. There are four human SALL proteins (SALL1, 2, 3, and 4) with structural homology and playing diverse roles in embryonic development, kidney function, and cancer. The SALL4 gene encodes at least three isoforms, termed A, B, and C, through alternative splicing, with the A and B forms being the most studied. SALL4 can alter gene expression changes through its interaction with many co-factors and epigenetic complexes. It is also known as a key embryonic stem cell (ESC) factor.
CITATIONS
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Chen Lingfeng,et al
Discovery of LC-MI-3: A Potent and Orally Bioavailable Degrader of Interleukin-1 Receptor-Associated Kinase 4 for the Treatment of Inflammatory Diseases
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WB
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Mouse
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Citation
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