PRODUCT CODE: ET7108-22

SAE1 Recombinant Rabbit Monoclonal Antibody [JG35-88] (ET7108-22)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of SAE1 on different cell lysates using anti-SAE1 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: SiHa   <br />
         Lane 2: K562<br />
 Lane 3: 293
  • Western blot analysis of SAE1 on different cell lysates using anti-SAE1 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: SiHa   <br />
         Lane 2: K562<br />
 Lane 3: 293
  • Flow cytometric analysis of A549 cells with SAE1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of SAE1 on different cell lysates using anti-SAE1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: SiHa
Lane 2: K562
Lane 3: 293

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

SAE1 Recombinant Rabbit Monoclonal Antibody [JG35-88] (ET7108-22)

Immunogen

Recombinant protein within human sae1 aa 150-300.

Host

Rabbit

Positive Control

SiHa, K562, 293, A549.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG35-88

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

SAE1

SYNONYMS

Activator of SUMO1 antibody; AOS1 antibody; HSPC140 antibody; Sae1 antibody; SAE1_HUMAN antibody; Sentrin/SUMO activating protein AOS1 antibody; SUA1 antibody; SUMO 1 activating enzyme E1 N subunit antibody; SUMO 1 activating enzyme subunit 1 antibody; SUMO-activating enzyme subunit 1 antibody; Ubiquitin like protein SUMO1 activating enzyme antibody; Ubiquitin-like 1-activating enzyme E1A antibody; UBL E1A antibody; UBLE1A antibody

SEQUENCE SIMILARITIES

Belongs to the ubiquitin-activating E1 family.

TISSUE SPECIFICITY

Expression level increases during S phase and drops in G2 phase (at protein level).

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Proteolytic degradation by the ubiquitin (Ub) system is essential for normal cell cycle progression, cellular differentiation and stress responses. Proteins conjugated to Ub are marked for progressive degradation by the 26S Proteasome. AOS-1, also designated SUMO-1-activating enzyme or ubiquitin-like 1-activating enzyme E1A, belongs to the ubiquitin-activating E1 family of proteins and plays an important role in the first step of the UBL1 conjugation pathway. AOS-1, which is a dimeric enzyme, functions as a UBLI E1 ligase, mediating the ATP-dependent activation of UBL1. AOS-1 can bind with UBLE1A and UBLE1B to form a heterodimer which can bind UBL1.