S100A10 Recombinant Rabbit Monoclonal Antibody [JF0987] (ET1702-38)
Catalog# ET1702-38
Rabbit Monoclonal to S100A10
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
Western blot analysis of S100A10 on different lysates with Rabbit anti-S100A10 antibody (ET1702-38) at 1/1,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD S100A10 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-38) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
S100A10 Recombinant Rabbit Monoclonal Antibody [JF0987] (ET1702-38)
Immunogen
Synthetic peptide within human s100a10 aa 19-68 / 97.
Host
Rabbit
Positive Control
A549 cell lysate, human lung tissue lysates, human lung tissue, human colon cancer tissue, human colon tissue, mouse colon tissue, rat colon tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF0987
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IHC-P
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1:1,000-1:2,000
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IF-Tissue
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 11 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
S100A10
SYNONYMS
42C antibody;AA409961 antibody;AL024248 antibody;Annexin II ligand antibody;Annexin II ligand, calpactin I, light polypeptide antibody;Annexin II tetramer (AIIt) p11 subunit antibody;Annexin II, light chain antibody;ANX2L antibody;ANX2LG antibody;Ca[1] antibody;CAL12 antibody;CAL1L antibody;Calpactin I light chain antibody;Calpactin I, p11 subunit antibody;Calpactin-1 light chain antibody;Cellular ligand of annexin II antibody;CLP11 antibody;GP11 antibody;MGC111133 antibody;Nerve growth factor-induced protein 42C antibody;OTTHUMP00000015269 antibody;OTTHUMP00000015270 antibody;p10 antibody;p10 protein antibody;p11 antibody;Protein S100 A10 antibody;Protein S100-A10 antibody;S100 calcium binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11)) antibody;S100 calcium binding protein A10 (calpactin) antibody;S100 calcium binding protein A10 antibody;S100 calcium-binding protein A10 antibody;S100a10 antibody;S10AA_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the S-100 family.
SUBCELLULAR LOCATION
Extracellular exosome.
FUNCTION
The S-100 family of calcium-activated proteins interact with a range of target proteins to modulate biological signaling pathways. Numerous cancer cell lines overexpress the plasminogen receptor S-100A10 on the extracellular cell surface, where it forms a heterotetrameric complex with Annexin II, though this association is not required for plasma membrane localization or binding and activation of plasminogen. Additionally, S-100A10 acts as a cellular chaperone for hepatitis B (Hep B) virus polymerase. Hep B virus polymerase normally localizes to the cytoplasm only, though in the presence of S-100A10 a portion relocates to the nucleus, implying a role for S-100A10 and intracellular calcium in the process of viral replication.
CITATIONS
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Jing Guo,et al
Orexin‐A Attenuates the Inflammatory Response in Sepsis‐Associated Encephalopathy by Modulating Oxidative Stress and Inhibiting the ERK/NF‐κB Signaling Pathway in Microglia and Astrocytes
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IF
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Mouse
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Citation
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