PRODUCT CODE: ET1703-72

S100 Recombinant Rabbit Monoclonal Antibody [JM51-31] (ET1703-72)

  • Recombinant

Applications

  • WB

  • IP

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of S100 on mouse heart cells lysates using anti-S100 antibody at 1/500 dilution.
  • Western blot analysis of S100 on mouse heart cells lysates using anti-S100 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- S100 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- S100 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- S100 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue using anti-S100 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with S100 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of S100 on mouse heart cells lysates using anti-S100 antibody at 1/500 dilution.

Applications

  • WB

  • IP

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

S100 Recombinant Rabbit Monoclonal Antibody [JM51-31] (ET1703-72)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse heart, SH-SY5Y, human tonsil tissue, human kidney tissue, mouse brain tissue, mouse spinal cord tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM51-31

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

11 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

S100

SYNONYMS

NEF antibody; Protein S100 B antibody; Protein S100-B antibody; S 100 calcium binding protein beta chain antibody; S 100 protein beta chain antibody; S-100 protein beta chain antibody; S-100 protein subunit beta antibody; S100 antibody; S100 calcium binding protein beta (neural) antibody; S100 calcium-binding protein B antibody; S100 protein beta chain antibody; S100B antibody; S100B_HUMAN antibody; S100beta antibody

SEQUENCE SIMILARITIES

Belongs to the S-100 family.

TISSUE SPECIFICITY

Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

The family of EF-hand type Ca2+-binding proteins includes calbindin (previously designated vitamin D-dependent Ca2+-binding protein), S-100 α and β, calgranulins A (also designated MRP8), B (also designated MRP14) and C (S-100 like proteins), and the parvalbumin family members, including parvalbumin α and parvalbumin β (also designated oncomodulin). The S-100 protein is involved in the regulation of cellular processes such as cell cycle progression and differentiation. Research also indicates that the S-100 protein may function in the activation of Ca2+ induced Ca2+ release, inhibition of microtubule assembly and inhibition of protein kinase C mediated phosphorylation. Two S-100 subunits, sharing 60% sequence identity, have been described as S-100 α chain and S-100 β chain. Three S-100 dimeric forms have been characterized, differing in their subunit composition of either two α chains, two β chains or one α and one β chain. S-100 localizes to the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. S-100 is also detected in almost all benign naevi, malignant melanocytic tumours and in Langerhans cells in the skin. Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100 α and β are present in a variety of other tissues, and calbindin is present in intestine and kidney.