RUNX2 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [SD208-0] (HA750295)
Catalog# HA750295
Rabbit Monoclonal to RUNX2
Tested for
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IF-Cell
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IF-Tissue
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IHC-P
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WB
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FC
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IP
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
☑ Relative expression (RE)
Western blot analysis of RUNX2 on different lysates with Rabbit anti-RUNX2 antibody (HA750295) at 1/10,000 dilution.
Lane 1: MDA-MB-231 cell lysate
Lane 2: Saos-2 cell lysate
Lane 3: LNCaP cell lysate (low expression)
Lane 4: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 2 minutes 24 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750295) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of RUNX2 on different lysates with Rabbit anti-RUNX2 antibody (HA750295) at 1/10,000 dilution.
Lane 1: MDA-MB-231 cell lysate
Lane 2: Saos-2 cell lysate
Lane 3: LNCaP cell lysate (low expression)
Lane 4: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 2 minutes 24 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750295) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.