Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of RRM1 on different lysates with Rabbit anti-RRM1 antibody (ET7107-72) at 1/500 dilution.
Lane 1: Mouse thymus tissue lysate (20 µg/Lane)
Lane 2: A431 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 90 kDa
Observed band size: 90 kDa
Exposure time: 1 minute;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-72) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Belongs to the ribonucleoside diphosphate reductase large chain family.
This gene encodes one of two non-identical subunits which constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. This gene is oriented in a head-to-tail configuration with the stromal interaction molecule 1 gene (STIM1), with the 3' end of STIM1 situated 1.6 kb from the 5' end of this gene.
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.