RPS6 Mouse Monoclonal Antibody [A6B9] (HA600084)
Catalog# HA600084
Mouse Monoclonal to RPS6
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (20 µg/Lane)
Lane 5: HCT 116 cell lysate (20 µg/Lane)
Lane 6: THP-1 cell lysate (20 µg/Lane)
Lane 7: NIH/3T3 cell lysate (20 µg/Lane)
Lane 8: PC-12 cell lysate (20 µg/Lane)
Lane 9: Human brain tissue lysate (40 µg/Lane)
Lane 10: Human kidney tissue lysate (40 µg/Lane)
Predicted band size: 29 kDa
Observed band size: 33 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600084) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
RPS6 Mouse Monoclonal Antibody [A6B9] (HA600084)
Immunogen
Recombinant protein within human rps6 aa 1-150.
Host
Mouse
Positive Control
HeLa cell lysate, MCF7 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, HCT 116 cell lysate, THP-1 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human brain tissue lysate, human kidney tissue lysate, HeLa, human breast carcinoma tissue, human pancreas tissue, mouse colon tissue, rat brain tissue, rat hippocampus tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A6B9
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:200
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IHC-P
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1:2,000
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein G affinity purified.
Molecular Weight
Predicted band size: 29 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
RPS6
SYNONYMS
40S ribosomal protein S6 antibody;Air8 antibody;NP33 antibody;Phosphoprotein NP33 antibody;Pp30 antibody;Ribosomal protein S6 antibody;RP S6 antibody;rps6 antibody;RS6 antibody;RS6_HUMAN antibody;S6 antibody;S6 Ribosomal Protein antibody
SUBCELLULAR LOCATION
Cytosol, Endoplasmic reticulum, Nucleus.
FUNCTION
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
CITATIONS
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Upregulation of glutamate transporter 1 by mTOR/Akt pathway in astrocyte culture during oxygen-glucose deprivation and reoxygenation
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WB
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Rat
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Citation
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