RPE65 Recombinant Rabbit Monoclonal Antibody [JM61-51] (ET1703-82)
Catalog# ET1703-82
Rabbit Monoclonal to RPE65
Tested for
-
WB
-
IP
-
IHC-P
-
IF-Tissue
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of RPE65 on different lysates with Rabbit anti-RPE65 antibody (ET1703-82) at 1/2,000 dilution.
Lane 1: Mouse eyeball tissue lysate
Lane 2: Rat eyeball tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-82) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse eyeball tissue lysate
Lane 2: Rat eyeball tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-82) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.