PRODUCT CODE: ET1703-82

RPE65 Recombinant Rabbit Monoclonal Antibody [JM61-51] (ET1703-82)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of RPE65 on mouse eyeball cells lysates using anti-RPE65 antibody at 1/500 dilution.
  • Western blot analysis of RPE65 on mouse eyeball cells lysates using anti-RPE65 antibody at 1/500 dilution.
Western blot analysis of RPE65 on mouse eyeball cells lysates using anti-RPE65 antibody at 1/500 dilution.

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

RPE65 Recombinant Rabbit Monoclonal Antibody [JM61-51] (ET1703-82)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse eyeball, rat eyeball cells lysates.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM61-51

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

61 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

RPE65

SYNONYMS

All-trans-retinyl-palmitate hydrolase antibody; LCA 2 antibody; LCA2 antibody; Leber congenital amaurosis antibody; mRPE 65 antibody; mRPE65 antibody; p63 antibody; rd 12 antibody; rd12 antibody; Retinal pigment epithelium specific 61 kDa protein antibody; Retinal pigment epithelium specific 65 kDa protein antibody; Retinal pigment epithelium specific protein antibody; Retinal pigment epithelium specific protein 65kDa antibody; Retinal pigment epithelium-specific 65 kDa protein antibody; Retinitis pigmentosa 20 antibody; Retinoid isomerohydrolase antibody; Retinol isomerase antibody; RP 20 antibody; RP20 antibody; RPE 65 antibody; RPE65 antibody; RPE65_HUMAN antibody; sRPE 65 antibody; sRPE65 antibody

SEQUENCE SIMILARITIES

Belongs to the carotenoid oxygenase family.

TISSUE SPECIFICITY

Retina (at protein level). Retinal pigment epithelium specific.

POST-TRANSLATIONAL MODIFICATION

Palmitoylation by LRAT regulates ligand binding specificity; the palmitoylated form (membrane form) specifically binds all-trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A) (By similarity).

SUBCELLULAR LOCATION

Cytoplasm. Cell membrane.

FUNCTION

The retinal pigment epithelium (RPE) is a monolayer simple epithelium in proximity to the outer surface of the retinal photoreceptor cells. Retinal pigment epithelium-specific protein (RPE65) is a 65kDa protein belonging to the beta-carotene dioxygenase family. This protein is important in 11-cis retinal production as well as in visual pigment regeneration. RPE65 is attached to the membrane by a lipid anchor when palmitoylated (membrane form) and soluble when unpalmitoylated. The soluble form of the protein binds vitamin A. Defects in RPE65 causes autosomal dominant retinitis pigmentosa and/or Leber congenital amaurosis type 2.